XB-CLONE-3063994
|
Clone Name: IMAGE:7845643
|
Gene:
rps6
|
Species: Xenopus tropicalis
|
|
|
|
Sequence:
5' EST: DT527926 
[+]
cttaacatctccttcccagccactggctgccaaaagctcatcgaagtggaggatgagcgcaagctgcgtaccttctatgagaagcgcatggctacagagg
ttgctgcagatcccttgggtgatgagtggaagggatatgtcgttcgcatcagcggtggaaatgataagcaaggctttcccatgaaacagggagtgctaac
tcatggccgtgttcgtcttctgttgagcaagggtcattcctgttatcgccccaggaggactggtgaacgcaagcgcaagtctgttcgtgggtgtattgtg
gatgctaacctgagtgtcctgaacttggttattgttaggaaaggcgagaaggatattcctggacttacagacaacactgttcctcgtcgcctgggtccca
aaagagccagcagaatccgcaaactgttcaacttgtcaaaagaagatgatgtgcgtcaatatgtagtgaggaagcctctggctaaggaggggaagaagcc
caggaccaaggcccctaaaatccagcgtctagtgaccccgagagttctgcagcacaagcgcagacgtattgctttgaagaagcagcgcactcagaagaat
aaggaagaggcatcagagtatgctaaacttctggctaagagaacaaaggaagccaaggaaaaacgccaggagcaaattgccaagaggcgcagactgtctt
ctttgagagcctccacatccaaatctgaatcgagtcagaaataaaactccatcatgtaaaaataaatacattttgttgtaaactta
3' EST: DT527925 [+]agtttacaacaaaatgtatttatttttacatgatggagttttatttctgactcgattcagatttggatgtggaggctctcaaagaagacagtctgcgcct
cttggcaatttgctcctggcgtttttccttggcttcctttgttctcttagccagaagtttagcatactctgatgcctcttccttattcttctgagtgcgc
tgcttcttcaaagcaatacgtctgcgcttgtgctgcagaactctcggggtcactagacgctggattttaggggccttggtcctgggcttcttcccctcct
tagccagaggcttcctcactacatattgacgcacatcatcttcttttgacaagttgaacagtttgcggattctgctggctcttttgggacccaggcgacg
aggaacagtgttgtctgtaagtccaggaatatccttctcgcctttcctaacaataaccaagttcaggacactcaggttagcatccacaatacacccacga
acagacttgcgcttgcgttcaccagtcctcctggggcgataacaggaatgacccttgctcaacagaagacgaacacggccatgagttagcactccctgtt
tcatgggaaagccttgcttatcatttccaccgctgatgcgaacgacatatcccttccactcatcacccaagggatctgcagcaacctctgtagccatgcg
cttctcatagaaggtacgcagcttgcgctcatcctccacttcgatgagcttttggcagccagtggctggga
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-ACTAGTGCGGCCGCCTAGGCCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 1.5 kb. The original library contained 1E5 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).| Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI |  |
Usage:
Suppliers:
