XB-CLONE-3176434
Clone Name: IMAGE:7854748
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Gene:
hspa1b
,
hspa1l
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Species: Xenopus tropicalis
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Sequence:
5' EST: DT396826
[+]
tgattctctctttgaaggtatagacttttacacctcaatcaccagggctcgctttgaggaactgtgcgcagatctgttcaggggcaccctggaacctgtg
gagaaagccatgagggatgccaaactagacaagtcccagatccatgagatcgtcctggtgggaggttcaactcgcattcccaaagtccagaagctgctgc
aagatttctttaatggcaaagagctgaacaaaagcatcaatcccgatgaggcagtggcctatggggcagcagtacaagctgccatcttgatgggcgacaa
gtctgagaatgttcaagacctgctcttgttggatgtggctcctctgtcattgggtctggaaactgcaggaggtgtaatgactgttcttattaagcgcaac
actaccattcctacaaagcagacccagaccttcactacttattcagacaaccagccgggggttctgatccaggtgtatgaaggagaaagagccatgacca
aggataacaacctcctgggcaagtttgacctgactggcatccctccagctccccgaggtgtgccccagatagaggtaacctttgatattgatgccaatgg
aatcctcaatgtctctgctgttgacaagagctcagggaagcagaacaaaatcaccatcactaatgacaagggcaggctgagcaaggaggagattgagagg
atgctgcaggaggcagacaaatacaaagctgatgatgaacaacagagggagaaaattgctgctaagaactccctggaatcctacgcatttaactgaagag
cacagtggaggataataatattaaggacaaaatcagtgacaatgacaagaaatccatcctggacaaatgccaggaggtcatctcatggctggagca
3' EST: DT396825
[+]ttttaacaaaaagcacttttattttcaaaacatatattaaacgcatattaaactaaagtgtttaagtgcaaatagaatttactttaagcattgacatcaa
tacggtttggcacattttggtaaacaaagacaaagcatgctgcccacgcactgataatattgtccaaaaatgtatgatgagaaaaaattaaattagtcaa
cttcctcaatagttggcccttgtttgggctcagcagtgcagccagcccctggcattccccctggcattccccctggcatcccaccttggtacagtttggt
aataatgggattacacagcttttccagctctttctgcttgtgggtatattcctccttttctgcgagttggttctgctccagccatgagatgacctcctgg
catttgtccaggatggatttcttgtcattgtcactgattttgtccttaatattattatcctccactgtgctcttcagttaaatgcgtaggattccaggga
gttcttagcagcaattttctccctctgttgttcatcatcagctttgtatttgtctgcctcctgcagcatcctctcaatctcctccttgctcagcctgccc
ttgtcattagtgatggtgattttgttctgcttccctgagctcttgtcaacagcagagacattgaggattccattggcatcaatatcaaaggttacctcta
tctggggcacacctcggggagctggagggatgccagtcaggtcaaacttgcccaggaggttgttatccttggtcatggctctttctccttcatacacctg
gatcagaacccccggctggttgtctgaataagtagtgaaggtctgggtctgcttttgt
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-ACTAGTGCGGCCGCCTAGGCCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 1.5 kb. The original library contained 1E5 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI |  |
Usage:
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