XB-CLONE-3178132
Clone Name: IMAGE:7853876
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Gene:
myl1
,
slc25a4
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Species: Xenopus tropicalis
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Sequence:
5' EST: DT395703
[+]
taataaaactttatttttttgaatacagaaataaaataatttaaccactccactatcagaagggccagttactggaacctctggtagtgcagaattgcag
tgatcttgaaaaactggcccaaagcctttggcaggttaaataaaaagacttggtagccaccaaattagcaaaggtgtttattgacatgtctttttttttt
tttacagtgctccatggtatggtacacagatctgcctagaagatttcgccaccaactttgtcgggggttgcatcgtcctgtttgatccgttttaaatatt
ggcagggttcatgaaagtaaatgttgaaattccagtgatgaaaaataaaaaaaaaatggtgggtgtattgtacagttcagttccgtatagttagttgtaa
ccagttgctggtttttgtacagttctggtgctcccggtgttctgagacattcttgtgacagttcttgagatatccgcttagacggacatgatgtgtttca
caaaggcttcatagttgatacagccgttggagtcttcctgacctgccagaagagcttctacttcctcctccctcatcttctcacccagagtggccagtac
gtgacgaagctcagcacccataacagtgccgttgccttccttgtcaaagacacggagaccctcaacaaagtcttcaaaggagccctggtccttgttgttg
gcaacggcctgcatcatgggcaggaactgctcaaactcaatcttcttggcgttcatctcttcagcagaggggttgcccaggaccttcttgacctcagcat
ttgtggggttctgtcccagagctctcatcacatcagctacctggttgagggcaattttgctgtctcctgtcctgtcaaagag
3' EST: DT395702
[+]tttttttaaatttaaatgttatctttattgtccacaatcttaagaaaagtctgccactgtcacggtattgggtaaaaatggctattatagcctttagaag
gtctattttattcattatgtttaggagcatggccatgttttgtacctagtgatggtgcacaggttgcagtgaacttcatttgtctatacaaaatgcaaac
tgggttgaagaatcccatttctccatgtttctccttttatttccctaggaaatatctcattgtctttgtcactgaaaccaaatgccccattatgtcttta
caaagtatatgaatagggagagaatatagcagttgtataagattagacatatttcttaatttcatcatacaatacaagtacaaaagcaccacccatgcct
ctcagtacgttggaccaagcacccttgaagaaggcttttggtccttcatctttggatatcttcttccagcagtcaatcgttcccttgtacatgatatcag
ctcctttcctgccagactgcatcatcatacgtctcctgactgtgtcaaatggataggacacaagaccagcaacagcagtgacagtctgagcaatcatcca
gcttaccacaatgtgcacattcttgggatctggcatcatacccttagcagtgtcatagacaccaaagtaagctgctctgtaaatgataatgccctgaaca
gacacattgaaaccttggtagaggccttttaatccatctaacttgtagatcttggcaatgcagtttccaagaccggtaaactctctttcatttagacctt
tgcccacatcagcggctagacgggttctggcaaaatccagggggtacacaaagcagagagatgtagcaccagcagctccaccagaggccag
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-ACTAGTGCGGCCGCCTAGGCCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 1.5 kb. The original library contained 1E5 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI |  |
Usage:
Suppliers: