XB-CLONE-3178887
Clone Name: IMAGE:7854047
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Gene:
etfa
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Species: Xenopus tropicalis
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Sequence:
5' EST: DT395203
[+]
cagaaggggactccatctctgcgcaggttccttagtgccctggttattgcagagcatgacacccaacaggtgacaccggtcactctcagcgccataactg
cagccaagcgacttggggcagatgtatcctgtttggtggcagggaccaactgcagtaaggtagcagaggagctggcaaaggtacagggagtgtcaaaact
gttcgtagcacagcatgaatcatacaagggatttttaccagaggagctgactccattggttttggaagcacagaagaagttcaatttcactcacatttgt
gccggagcatcagcgtttggaaagagtcttctaccaagaattgctgcaaagcttgatgtggctcctgtatctgatgtcattgagattaaatctccagata
catttgtgaggaccatctatgctggtaatgctttatgtactgtgaaatgtgaggagtctgtcaaggtgttttctataagagggacatcctttgaggctgc
tgaaacatcaggaggaagtgcaagctcagacacagtggctccatctgcaccagctggactatctgagtggcttcatcagagtttgacaaaaagcgaccgt
ccggagctgactagcgccaaggttgttgtatctggaggccgaggtttaaagagtggagaaaatttcaagctgttatatgatctggcagatcagctgaatg
ctgcagttggcgcttcccgtgctgctgtggatgctggctttgtacccaatgacatgcaggtgggacagacaggcaaaattgttgctcctgaactgtacat
tgctgttggaatctctggagctattcagcaccttgctgggatgaaagatagcagactattgttgctataaac
3' EST: DT395202
[+]ttttttgttacagcaaatatttaatccagaattttgaagcagcagaagtattgctggaacacatcagtcgcagcttatacaaaaataatttgccaacatt
ccattactttgcagggatcagtgtgctgtgggtgagtgcggcacttcagtctatggctggatcactgatgattaaacaagcatctcttattcatccaagt
gcagaagtcactttttcttaagtatttcagtcatttctggcactgccttgaacaagtctgccaccaaaccatagtcagccacctggaagattggtgcctc
aggatccttgtttatagcaacaatagtcttgctatctttcatcccagcaaggtgctgaatagctccagagattccaacagcaatgtacagttcaggagca
acaattttgcctgtctgtcccacctgcatgtcattgggtacaaagccagcatccacagcagcacgggaagcgccaactgcagcattcagctgatctgcca
gatcatataacagcttgaaattttctccactctttaaacctcggcctccagatacaacaaccttggcgctagtcagctccggacggtcgctttttgtcaa
actctgatgaagccactcagatagtccagctggtgcagatggagccactgtgtctgagcttgcacttcctcctgatgtttcagcagcctcaaaggatgtc
cctcttatagaaaacaccttgacagactcctcacatttcacagtacataaagcattaccagcatagatggtcctcacaaatgtatctggagatttaatct
caatgacatcagatacaggagccacatncagctttgcagcaattcttggt
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-ACTAGTGCGGCCGCCTAGGCCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 1.5 kb. The original library contained 1E5 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI |  |
Usage:
Suppliers: