XB-CLONE-3183958
Clone Name: IMAGE:7852245
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Gene:
slc43a2
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Species: Xenopus tropicalis
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Sequence:
5' EST: DT391849
[+]
catcgattcgtttttctgaggactttcctttgggtggagccagagaaactcgattttgggacgaaatctgcaaaataagatggctgttcatttcatgttc
ccttcacccattcttggcctggagttttcccattttaactttcttagcattttctgtgaatgcttttcttagcatttctgtgatttgttttttttacttt
ctttccaaacattatagtattcactttcctttttgtgactttacacttatgtttaattctgtcatattctatatctgtagaattctgttgcatcgttcta
tttctgctgcttcactatattatcccttatttaagctgttattcttaggggtttaacattacctcactcccatcatccctatctagtgtctttctggatt
aagttacacagctggcaaaacccatatctgtgtgggacctgactgagtgacctgggtgatctgagatgaatccaggaacgcaaataagtccttcttgcac
ataacaggtttcaggaagtgtagacttcctgcataactacacaagcattcgctcactggcacggcctgcagaaccacaaatacgcattggaaaatttgtt
ttcaggctggaggcaattcagctctaaaacagtatctgcctttatgtttgataccaatcatctgacttggaaatggccaaatccttctcacagtgctctc
atgttaaaataggcagagaactgagagggaattgccagaatattagctggaagtagagagccgttttgttgtaaatcccaggttatgtgatggcagaatg
catcttctgctggttaatagacattaagaggaaatctatagcacannaagcatat
3' EST: DT391848
[+]cacagtacagatggtagtttaatgtaaaaaggatttattctcagaagtaccaacaatcactgtatgtagaatgtacaggcagacgatacattaaactcca
aagagcttacaatctgtttgtacctgaggcactgtgtaatacagtgatgtgcccaagtttacactgagctggcactgggctttagcactgataccttatt
ctggttattcttccaagcctactttctgttagacataacaaaggaaacatttataatatatttcttttttatattgcttttgtgctatagatttcctctt
aatgtctattaaccagcagaagatgcattctgccatcacataacctgggatttacaacaaaacggctctctacttccagctaatattctggcaattccct
ctcagttctctgcctattttaacatgagagcactgtgagaaggatttggccatttccaagtcagatgattggtatcaaacataaaggcagatactgtttt
agagctgaattgcctccagcctgaaaacaaattttccaatgcgtatttgtggttctgcaggccgtgccagtgagcgaatgcttgtgtagttatgcaggaa
gtctacacttcctgaaacctgttatgtgcaagaaggacttatttgcgttcctggattcatctcagatcacccaggtcactcagtcaggtcccacacagat
atgggttttgccagctgtgtaacttaatccagaaagacactagatagggatgatgggagtgaggtaatgttaaacccctaagaataacagcttaaataag
ggataatatagtgaagcagcagaaatagaacgatgcaacagaattctaccga
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-ACTAGTGCGGCCGCCTAGGCCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 1.5 kb. The original library contained 1E5 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI |  |
Usage:
Suppliers: