XB-CLONE-3184140
Clone Name: IMAGE:7851989
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Gene:
myl12b
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Species: Xenopus tropicalis
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Sequence:
5' EST: DT391728
[+]
ccgagggggaagtgaaattgcacgaataacccaccgccaagatgtccagcaaaagagcaaagaccaagaccaccaaaaagcgcccccagcgcgcaacttc
taatgtatttgccatgtttgaccagtcccaaatccaggagtttaaggaggcattcaacatgattgatcagaaccgtgatggctttattgataaagaagat
ctgcatgacatgcttgcctctcttggaaaaaaccctacagacgagtatttagatgccatgatgaatgaagcacctggccctattaacttcaccatgttcc
ttaccatgtttggggagaagctgaacggaaccgatcctgaggatgttattaggaatgcctttgcatgctttgatgaagaaggaataggttctatccagga
ggatactctgagggaattgctgacaacaatgggtgaccgatttacagatgaagaagtagatgagctcttcagagaggccccaatcgacaagaagggcaat
ttcaattacaatgaattcacccgcatcttgaaacatggagctaaagataaggatgactaagctggaagtgcccagtggtgtaagaacaactgcactgctc
tatatcatcctcacgttcctcttcctgatagaactcattgcatgcatttagctctagatcttttcttttctgtgtatttatggatctgaaccaggccctt
ttggcaaatgggcacatatgtaaccaaactggaaaacaaaaaaaagtgcaatgtaacacgcgttacacagcaggttttttcttgggacttgagctggatt
ttttgcaattttgtagtanaatttggaattaagataactgtcagttaaaacttttctccgaatggcctggcaaagtggaa
3' EST: DT391727
[+]aagttttagctgggatatgccacaagcagcttttattgagaagagtaaatgctacaactgtcagataaacaagtctataggagccaactgcatagctaac
cccagtactaaacagggcctatttttaacaccaacatatatatatattcacataggaatatcttatttccactttgccaggccattcggagaaaagtttt
aactgacagttatcttaattccaaattttactacaaaattgcaaaaaatccagctcaagtcccaagaaaaaacctgctgtgtaacgcgtgttacattgca
cttttttttgttttccagtttggttacatatgtgcccatttgccaaaagggcctggttcagatccataaatacacagaaaagaaaagatctagagctaaa
tgcatgcaatgagttctatcaggaagaggaacgtgaggatgatatagagcagtgcagttgttcttacaccactgggcacttccagcttagtcatccttat
ctttagctccatgtttcaagatgcgggtgaattcattgtaattgaaattgcccttcttgtcgattggggcctctctgaagagctcatctacttcttcatc
tgtaaatcggtcacccattgttgtcagcaattccctcagagtatcctcctggatagaacctattccttcttcatcaaagcatgcaaaggcattcctaata
acatcctcaggatcggttccgttcagcttctccccaaacatggtaaggaacatggtgaagttaatagggccaggtgcttcattcatcatggcatctaaat
actcgtctgtagggttttttcca
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-ACTAGTGCGGCCGCCTAGGCCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 1.5 kb. The original library contained 1E5 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI |  |
Usage:
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