XB-CLONE-3185217
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Clone Name: IMAGE:7851843
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Gene:
slc25a4
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Species: Xenopus tropicalis
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Sequence:
5' EST: DT391019 
[+]
ttttttttttctaaatttaaatgttatctttattgtccacaatcttaagaaaagtctgccactgtcacggtattgggtaaaaatggctattatagccttt
agaaggtctattttattcattatgtttaggagcatggccatgttttgtacctagtgatggtgcacaggttgcagtgaacttcatttgtctatacaaaatg
caaactgggttgaagaatcccatttctccatgtttctccttttatttccctaggaaatatctcattgtctttgtcactgaaaccaaatgccccattatgt
ctttacaaagtatatgaatagggagagaatatagcagttgtataagattagacatatttcttaatttcatcatacaatacaagtacaaaagcaccaccca
tgcctctcagtacgttggaccaagcacccttgaagaaggcttttggtccttcatctttggatatcttcttccagcagtcaatcgttcccttgtacatgat
atcagctcctttcctgccagactgcatcatcatacgtctcctgactgtgtcaaatggataggacacaagaccagcaacagcagtgacagtctgagcaatc
atccagcttaccacaatgtgcacattcttgggatctggcatcatacccttagcagtgtcatagacaccaaagtaagctgctctgtaaatgataatgccct
gaacagacacattgaaaccttggtagaggccttttaatccatctaacttgtagatcttggcaatgcag
3' EST: DT391018 [+]ggagcttcgcccgatccggccccaccatgagtgaccaggttttaagctttcttaaggactttctggcgggtggcattgcagctgccatctctaagactgc
tgtagcccccattgaaagggttaaacttctgctgcaggtccagcatgccagcaaacagatatctgttgacaagcaatataaagggatcatggattgtgtg
gtcagaatcccaaaggagcaaggatttatctcattctggagagggaaccttgccaatgtcatccgttacttccccacacaagctcttaactttgccttca
aggacaagtataagcagattttccttgggggagtagataagcataagcaattctggaggttcttcgttggtaacctggcctctggtggagctgctggtgc
tacatctctctgctttgtgtaccccctggattttgccagaacccgtctagccgctgatgtgggcaaaggtctaaatgaaagagagtttaccggtcttgga
aactgcattgccaagatctacaagttagatggattaaaaggcctctaccaaggtttcaatgtgtctgttcagggcattatcatttacagagcagcttact
ttggtgtctatgacactgctaagggtatgatgccagatcccaagaatgtgcacattgtggtaagctggatgattgctcagactgtcactgctgttgctgg
tcttgtgtcctatccatttgacacagtcaggagacgtatgatgatgcagtctggcaggaaaggagctgatatcatgtacaagggaacgattgactgctgg
aagaagatatccaaagatgaaggaccaaaagccttc
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-ACTAGTGCGGCCGCCTAGGCCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 1.5 kb. The original library contained 1E5 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).| Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI |  |
Usage:
Suppliers:
