XB-CLONE-3188271
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Clone Name: IMAGE:7850659
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Gene:
eif2s3
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Species: Xenopus tropicalis
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Sequence:
5' EST: DT389000 
[+]
gacttggccacattgaatgtttctaagctgacaccgctctcgccagaaatcatcagccggcaagccacaattaacataggcaccataggtcacgtagccc
atggaaaatcaactgtagttaaagctatttctggggttcacactgtccgatttaaaaatgagctggagagaaatattaccatcaagctgggatatgccaa
tgccaagatttaccaacttgatgatcctagctgttctcggcctgaatgctatcgatcgtgtgggagcagcactccagatgagtttccaactgatatccct
aacaccaagggcaactttaaattggtcagacatgtttcttttgtggattgcccaggccacgatattttgatggctactatgttaaacggtgccgctgtta
tggatgcagctttattgttaatagctggtaatgagtcttgtccacagcctcagacatcagaacacctggctgctatagaaatcatgaaattaaaacacat
actaattttacaaaacaagattgatttggtcaaagaaagtcaagcaaaagaacagtatgagcagatccttgcatttgtacaaggtacagttgcagaagga
gcacctatcattccaatctctgctcagctaaagtacaatattgaagtagtttgcgaatacatagtaaagaaaatccctgtgcccctacgagacttcatct
ctgagcccaggctcattgttattagatcttttgatgtcaacaagccaggatgtgaagttgatgaccttaaaggtggtgtggctggnggcagtattcttaa
aggtgtat
3' EST: DT388999 [+]ggtcttttatagtttattttattgacatttatttatgggtgaagtcctgaagccaatgggcacttcagggaaaatgttactctgtaacaaaagcatacaa
tttgaggagttacatgccaaaaggcatttgtagaaagcgaagagcaattcccagactgagaacaactccactataaattagaaaatgggatcgtaaatgt
aaatgtctggctgcagtgatttagtaaatccaattcctgaataagtaaagggcatgtttcttctaaaaacatttcataatgctggtgtctgcttgacggc
tagtcatcatctactgttggcttgattgtcacacctcgtctaatctggccccatccaatcaaacgccagtgcttttccactcttcggctaagggcaattt
tctctcctacttctgtgcacactgggtttgtcaggacaattttccctaaatcagctttgactgcactaacacgaccgccagtggacaaagagccaatgtt
gaccatcagaacctcattcttggacagcttttgtacctttgcagccttcttatccccttcaatgcgcacaccaagaagtcgtcgcagaaggaagtaggag
atttccagctctgtgaagatttcaggcagagcacccactgctcctaaaacctgacccaccatacgatcagcacggcacaatgttggatcaatcttggtac
caacaccaataaggcccccaggagctgcatattgtagatcattgtgttctgcaaacaaagacacaattttggaaaatattggcttgcacatgagtttccc
ttcactgtcttttgagac
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-ACTAGTGCGGCCGCCTAGGCCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 1.5 kb. The original library contained 1E5 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).| Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI |  |
Usage:
Suppliers:
