XB-CLONE-3189979
Clone Name: IMAGE:7849740
|
Gene:
pdlim3
|
Species: Xenopus tropicalis
|
|
|
Sequence:
5' EST: DT387868
[+]
ctgggatgtagggcaactgaacaactggtcattctgttctttcattagagattttgtttttctccgcatttttataattttccaggtaaatgtgtcttgt
tttcttctacatattgagtagatacatttttcatattataggaaagagtttgttagtgtgctggtgccttcccctttaagaatacagagattgttggctc
atttattgcagtacattaagttggctgaatattatattttgacccatgcaactacatccaacttgaaaggtgagttctgtcgatccagcaccatcctaca
aaatctcccatgtagtttagctcttatggacacagaatctctgttttatttaaaaggaaagagaataattatcttggtagtttttggccaaaatgtctta
atgtcctttaacatatttatatttagttgctgtaggaacacatccatttgtcactagagggagtgttatgttgtccttatccattctttgctatcaatgt
ataacaatagaatataaacatgctagaagtttggaatcatgcacaagcttattttcatttttgcatttaatagaatacgtttgtattgtagtaatggaga
gcacaaagcacaatcgtatacatgagacaaaggacacgtctgttactcactagctgttgtccactagtattagtggtgggatgacatactcttggttgtt
actggggatatctgtagctttgaaaggcaaactgcatctggagggctgccagcttcccttgcctaagctatagtatttagctgggacgcanaagaacatt
atgggataagacggcctgttgtggacctcacacttttagtaccaacagccaatccagtaaatcatttaaaacatgttgtcatatatg
3' EST: DT387867
[+]ttgtagatttttcccccattatataacaaaataatagatgttcattggaaatggctgtgagcctagaaaggctatgaaaacaatataagcattaactata
ttagaacagatacagttaatagtgcatactgcagttcaaacacagtgaaaacatatgtgaacacattctctaacaataatacacagtactcaatacacaa
tgtttcaaactatgtttctgattggaagcccagtgacttaatgacactttggcagtcacaaggcattagccagtataacatgtaaagtggcactgtgtat
gcattacaagtacagttttaagtccaaagtaaaatattttagtccatcagtaatgctgttctcctctcacacaattctcatatatgacaacatgttttaa
atgatttactggattggctgttggtactaaaagtgtgaggtccacaacaggccgtcttatcccataatgttcttttgcgtcccagctaaatactatagct
taggcaagggaagctggcagccctccagatgcagtttgcctttcaaagctacagatatccccagtaacaaccaagagtatgtcatcccaccactaatact
agtggacaacagctagtgagtaacagacgtgtcctttgtctcatgtatacgattgtgctttgtgctctccattactacaatacaaacgtattctattaaa
tgcaaaaatgaaaataagcttgtgcatgattccaaacttctagcatgtttatattctattgttatacattgatagcaaagaatggataaggacaacataa
cactccctctagtgacaaatggatgtgttcctacagncactaaatataaatatgttaaaggacattaagacattttggccaanaactaccaagataatat
tctcttntcttttaaataa
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-ACTAGTGCGGCCGCCTAGGCCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 1.5 kb. The original library contained 1E5 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI |  |
Usage:
Suppliers: