XB-CLONE-3241437
|
Clone Name: IMAGE:7708905
|
Gene:
ube2s
|
Species: Xenopus tropicalis
|
|
|
|
Sequence:
5' EST: CX962609 
[+]
ctagcggagtgggagtgaggcgtgcctacaagcagccgaaggttatatgaaggggcgtcgaattacagttaggcctggactgttcgtacccagcctcaga
gaatcaaacaccgtcacggcgctgaacagttctcctccgtcccccgtttggcacagagaggcatgaattcaaacgttgaaaacttgcccccacacatcat
tcgtcaggtctataaagaagtctccactctgacatctgaccctccagaaggaataaagatcattccaaatgaagaagatataactgatgtccaagttaac
attgaaggaccagaaggcactccatatgcaggaggtatctttcgaatgaagctgattttgggcaaagacttcccagcagcaccaccaaagggatacttcc
taacaaagatattccatccaaatgttagcactaatggagagatctgtgtaaatgtgctaaagaaagactggaaagctgagctaggcataaggcatgtttt
gctgacaataaagtgtttgttgattcatccgaacccagagtcagcactgaatgaggaggcagggcgcctcttactggagaactatgaagagtacgcttcc
cgtgcaaggcttatgactgaaatccacgcccagggttccagcttaaggggcaaggatcctacagacccatgctcctctgcctcagccacacttgtcagtg
gggatggacccat
3' EST: CX962608 [+]tttttttttttttttaagaaaaagtattttaatagttttataagttaaataacttaagactgggaaaaactaacaaaagcaggcctttaaaaaacaaagc
gaggaaagaatatggattttaattgactccccaagtttcctcttgcgtcccacccacccagtttaaaagttcatatgcaagatccattcatgatctccaa
ccatccctcctgccctttggtaatgtttatcactggtgctaaagcctccggagagctctctttttgtctgtcttcttttttgctgcaagtttcttgtccc
tatctcctgcatgtttctttgccatgggtccatccccactgacaagtgtggctgaggcagaggagcatgggtctgtaggatccttgccccttaagctgga
accctgggcgtggatttcagtcataagccttgcacgggaagcgtactcttcatagttctccagtaagaggcgccctgcctcctcattcagtgctgactct
gggttcggatgaatcaacaaacactttattgtcagcaaaacatgccttatgcctagctcagctttccagtctttctttagcacatttacacagatctctc
cattagtgctaacatttggatggaatatctttgttaggaagtatccctttggtggtgctgctgggaagtctttgcccaaaatcagcttcattcgaaagat
acctcctgcatatggagtgccttctggtcccttcatgttaacttggacatcagttatatcttcttcatttggaatgatctttattccttctggagggtca
gatgtc
Library:
Description: PolyA+ RNA was primed with oligo-dT primer 5'-GACTAGTTCTAGATCGCGAG CGGCCGCCCTTTTTTTTTTTTTTT-3'. cDNA was ligated to SalI adapter (5'-TCGACCCACGCGTCCG-3' and 5'-CGGACGCGTGGG-3'), digested with NotI, size fractionated in 1.1% agarose gel electrophoresis and ligated into SalI and NotI digested pCMV-SPORT6 vector. Library contains the 2-3 kb size fraction (additional size fractions contained in the NIH_XGC_tropTe3 and Te4 libraries). Library constructed at the DOE Joint Genome Institute.| Anatomy: testis | Stage: frog |
| Normalized: No | Subtraction: No |
| Vector Details | Vector Map |
Name: pCMV-SPORT6
Type: phagemid
5' Restriction Site: SalI
3' Restriction Site: NotI |  |
Usage:
Suppliers:
