XB-CLONE-3242944
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Clone Name: IMAGE:7706818
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Gene:
nelfa
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX959223 
[+]
agaaagtcagtgaatgtgaagcatctgccatgctgccacttgaatgccagtacctcaacaagaatgccctgaccaccctagctggacccctcacgccccc
agtgaaacatttccagctcaaaagaaaaccaaaaagtgccacactcagagcagagctcttacagaaatcaacagaaactgcacagcagctgaagaaaacg
gcaggggttccatttcactcaaaaggacgaggcttggtaaaaaaactggacacaacaactcctctcaaaggtataccaaagcaggcacccttcaggagtc
caaccacaccaagtgtatttagtccgaccgctaacagaacacctatagcgccctccagaacgcccttgcgcaaggagagaggtgtaaagttactagacat
atctgagctggacacggtcggtggtggtcgggaagcgaagaagagaaggaagactgtagaagcagaagttgttgaaaaaccaccaaaggaggagactgta
ttggaaaaccctactccagactatgctgctggccttgtctctacacagaaactgggtgccttgaacaatgagagtgcacttccatctacaagctatttgc
cagcaactcccagcgtggtgccatcctcctcctatattccaacgtctgaagcacagcaagccggaccagtacgggaagccttgcaagcaacaaggcagcc
agaggagcccacagcagccaacactactcttccaacacagtttaaacaaaggacgccaatgtaccatagcaatccaaatgctgccaccactaccccttct
cctgcagc
3' EST: CX959222 [+]tttttttttttttttggtccacaggagaattctgggaattccttaagagacattggttattggcttgtacttcttgagcttggtccactggccagtagaa
tagttcatttcaaacactgtgtcgactaacatagtagtgctgcctgtaccgtctgcctttggcaattgctctgtgtattcgctgagtttgatttgaatga
tatcaccttgttctgggcaaggattctctctggatccagccataaagccaagaataagagccttctctggccttgtgactttgtttgccgtcttgaacat
ctcctgtgcggcgtacatctgctccctcgtgagagaaaggttcttcttgggttgcggctgcgtttgagtggttatctggggagcctgtgccgctggggtt
gctgcctgagtggcagcaggagttaatggagaggtaggggtcgctgcaggagaaggggtagtggtggcagcatttggattgctatggtacattggcgtcc
tttgtttaaactgtgttggaagagtagtgttggctgctgtgggctcctctggctgccttgttgcttgcaaggcttcccgtactggtccggcttgctgtgc
ttcagacgttggaatataggaggaggatggcaccacgctgggagttgctggcaaatagcttgtagatggaagtgcactctcattgttcaaggcacccagt
ttctgtgtagagacaaggccagcagcatagtctggagtagggttttcaatacagtctcctccttt
Library:
Description: PolyA+ RNA was primed with oligo-dT primer 5'-GACTAGTTCTAGATCGCGAG CGGCCGCCCTTTTTTTTTTTTTTT-3'. cDNA was ligated to SalI adapter (5'-TCGACCCACGCGTCCG-3' and 5'-CGGACGCGTGGG-3'), digested with NotI, size fractionated in 1.1% agarose gel electrophoresis and ligated into SalI and NotI digested pCMV-SPORT6 vector. Library contains the 2-3 kb size fraction (additional size fractions contained in the NIH_XGC_tropTe3 and Te4 libraries). Library constructed at the DOE Joint Genome Institute.| Anatomy: testis | Stage: frog |
| Normalized: No | Subtraction: No |
| Vector Details | Vector Map |
Name: pCMV-SPORT6
Type: phagemid
5' Restriction Site: SalI
3' Restriction Site: NotI |  |
Usage:
Suppliers:
