XB-CLONE-3248130
Sequence:
Full length sequence: BC166228 
[+]
gaaaaacccagcaggacagagagctgtgcaggggaaagaagatgcagggattcctgtcagagggaacatggatcgtaccggcaagtgtggagttaatgga
gcagtacatcagggtaggggcccatttctggccagactataggggtgcgggcacataccatgagcaggagaggaggaggatcagagaggagctgcagcag
gactatgtatagtgaagccttcctgcatcttggctttagtctgcatgttctagaagggcaatgagagatgtggaaggtctgagaggctttaacggaggca
caaagtggtgaccaactgccccactgagcacttgtgacattcagatcttcatgtccaggatttctggagtctcctgattaaagaaaagaaagagatgaag
cccttaccttgccgaagcaagagaaacagcacttcagccgcttgaacatgttgtttctatgaagacaaaatgaaaaacatcactttagcatttcccaata
cagctgtgaattgaaaaaggctggaaaatatggacttttcaccccaccgtggcatgcatgaaggaaggtctccttcccttcaagataccaccatgagctc
cagccacacccagaagaaccgaggggactccactccgctgcctccaatcaatgagcgcctggctttcctgcggccgtccagagagctgttagagtattac
cgcaagaagatcgctgagtttgatgaggaacacgaggatctggtaaagcaactggagcaatacaaagctacctttgaggagcagggatcgtaccggcaag
tgtggagttaatggagcagtacatcagggtaggggcccatttctggccagactataggggtgcgggcacataccatgagcaggagaggaggaggatcaga
gaggagctgcagcaggactatgtatagtgaagccttcctgcatcttggctttagtctgcatgttctagaagggcaatgagagatgtggaaggtctgagag
gctttaacggaggcacaaagtggtgaccaactgccccactgagcacttgtgacattcagatcttcatgtccaggatttctggagtctcctgattaaagaa
aagcagaaagaaatgaagcccttaccttgccgaagcaagagaaacagcacttcagccgcttgaacatgttgtttctatgaagacaaaatgaaaaacatca
ctttagcatttcccaatacaggtctccttcccttcaagataccaccatgagctccagccacacccagaagaaccgaggggactccactccgctgcctcca
atcaacgagcgcctggctttcctgcggccgtccagagagctgttagagtattaccgcaagaagatcgctgagtttgatgaggaacacgaggatctggtaa
agcaactggagcaatacaaagctacctttgaggagcaggaacgttcagataaggtggccaagcggctgcaattgatggcgcagagatacgaggctctgga
gaaaaggcgcaacatggaggtagagggctataagactgacatcaagctgctgagacagcggttaaaggatgtagagaagcaactattcaaggtgacgctg
aacatcaggccagaccaagatcttgctattctggatgcagtgcggcagggcaacaaaaagacacaaaaatttcaaggggaactgagcctggagaatgaac
tgagaatcggatagagcacaagggagactgacggagctgccacactaaacaaggagccacagataaaggtctaccctggctctacctctaggattatata
gtgtgttcggcccaggaaggaagaatattggcggccatacacgttgccaagcgagctgatcttctcccggtatccccacctacgggtgggttatatcggg
cgaattcaggcttattagaacaagggatattagaacaacgaatcaagtacagtacctgtacttgatcccaactaagatataattaccccttattgggggc
agaacagccctattgggtgtatttaatggttaaatgattcccttttctctgtaataataaaacagtacctgtacttgatcccaactaagatataattacc
ccttattggggcagaacagccctattgggtttatttaatggttaaatgattcccttttctctgtaataataaaacagtacctgtacttgatcccaactaa
gatataattaccccttattggggcagaacagccctattgggtttatttaatggttaaatgattcccttttctctgtaataataaaacaggacctgtactt
gatcccaactaagatataattaccccttattggggcagaacagccctattgggtttatttaatggttaaatgattcccttttctctgtaataataaaaca
gtacctgtacttgatcccaactaagatataattaccccttattgggggcagaacagccctattgggtttaaatgatgtttaaataatttttttagtaaat
ttaatgtatggagatccgaattacggaaagatcccttttccgaaaaaagccccaggtcctgagcattctggataacaggtcctatacctgtgcttagctt
tttgtacagcagctctccagtttggaaccttagctatcttgttgccagggcctttttaccttagcaactaggcagtgatttaaatgagaaacaggaatat
gaataggagagggtctgaatagaaggataaaaaataaagttcacagagcaatagttttttgttttgtttttttatctactgggtcagtgacccccattta
aaagcttgaaagaatcagaagaggaaggcaaataattcaaaaactaaactctggctggaaatgctgtattttatttactgaactccctgtaccagcccag
agagtcagcagccctatgaactaatgatcctggcctttaactttgtccacagcaggtctgtgtgtcagtggcactgcacatgctcagtgggctctaggct
gctgttgtgcagatatttacctttatattgtgagttggtccctaaggtaagtgtagtggagatcacacaaaactgttcctgtgagaccgagctagttaat
gataggggtttaaattgcttaatgctgaatttacacaattttgttttcaatcatgaaggcctgtgaatgtgttacttcaattgttatgattatcctcatt
atcttgtctttgtccaagttgctcttggccttgattatctcagctaatattcaaggctaagagatcataataaagcttcagcagctaattaaataatttc
tgtatttctactaataattgcaatgtacccacccatgatacgtaagcataatgggatgtgtttttctatctactatataagtaatatcaaaacaaaataa
agtcgtacttgtttgcaatacaaaaaaaaaaaaaaa
5' EST: CX923659 [+]aaaacccagcaggacagagagctgtgcaggggaaagaagatgcagggattcctgtcagagggaacatggatcgtaccggcaagtgtggagttaatggagc
agtacatcagggtaggggcccatttctggccagactataggggtgcgggcacataccatgagcaggagaggaggaggatcagagaggagctgcagcagga
ctatgtatagtgaagccttcctgcatcttggctttagtctgcatgttctagaagggcaatgagagatgtggaaggtctgagaggctttaacggaggcaca
aagtggtgaccaactgccccactgagcacttgtgacattcagatcttcatgtccaggatttctggagtctcctgattaaagaaaagaaagagatgaagcc
cttaccttgccgaagcaagagaaacagcacttcagccgcttgaacatgttgtttctatgaagacaaaatgaaaaacatcactttagcatttcccaataca
gctgtgaattgaaaaaggctggaaaatatggacttttcaccccaccgtggcatgcatgaaggaaggtctccttcccttcaagataccaccatgagctcca
gccacacccagaagaaccgaggggactccactccgctgcctccaatcaatgagcgcctggctttcctgcggccgtccagagagctgttagagtattaccg
caagaagatcgctgagtttgatgaggaacacgaggatctggtaaagcaactggagcaatacaaagctaccttttgagagcagggatcgtaccggcaagtg
tggagttaatggagcagtacatcagggtaggggcc
3' EST: CX923658 [+]gcaaacaagtacgactttattttgttttgatattacttatatagtagatagaaaaacacatcccattatgcttacgtatcatgggtgggtacattgcaat
tattagtagaaatacagaaattatttaattagctgctgaagctttattatgatctcttagccttgaatattagctgagataatcaaggccaagagcaact
tggacaaagacaagataatgaggataatcataacaattgaagtaacacattcacaggccttcatgattgaaaacaaaattgtgtaaattcagcattaagc
aatttaaacccctatcattaactagctcggtctcacaggaacagttttgtgtgatctccactacacttaccttagggaccaactcacaatataaaggtaa
atatctgcacaacagcagcctagagcccactgagcatgtgcagtgccactgacacacagacctgctgtggacaaagttaaaggccaggatcattagttca
tagggctgctgactctctgggctggtacagggagttcagtaaataaaatacagcatttccagccagagtttagtttttgaattatttgccttcctcttct
gattctttcaagcttttaaatgggggtcactgacccagtagataaaaaaacaaaacaaaaaactattgctctgtgaactttattttttatccttctattc
agaccctctcctattcatattcctgtttctcatttaaatcactgcctagttgctaaggtaaaaaggccctggcaacaagatagctaaggttccaactgga
gagctgctgtacaa
Library:
Description: PolyA+ RNA was primed with oligo-dT primer 5'-GACTAGTTCTAGATCGCGAG CGGCCGCCCTTTTTTTTTTTTTTT-3'. cDNA was ligated to SalI adapter (5'-TCGACCCACGCGTCCG-3' and 5'-CGGACGCGTGGG-3'), digested with NotI, size fractionated in 1.1% agarose gel electrophoresis and ligated into SalI and NotI digested pCMV-SPORT6 vector. Library contains the 1.5-2 kb size fraction (additional size fractions contained in the NIH_XGC_tropTe3 and Te5 libraries). Library constructed at the DOE Joint Genome Institute.| Anatomy: testis | Stage: frog |
| Normalized: No | Subtraction: No |
| Vector Details | Vector Map |
Name: pCMV-SPORT6
Type: phagemid
5' Restriction Site: SalI
3' Restriction Site: NotI |  |
Usage:
Suppliers:
