XB-CLONE-3255331
|
Clone Name: IMAGE:7688250
|
Gene:
mib2
|
Species: Xenopus tropicalis
|
|
|
|
Sequence:
Full length sequence: BC161083 
[+]
gcaagctggaagagcaggcacaactgctctgcccctaaccacaagggatgcgtatctgcctggtgtctgttacgcatgaggaaataagggctaaataaac
ctggctgctttgtacactgtttggacatcaggcctctaagctttctgctctatcagtaaacttcttcaatgcctgcagtagttcagttgctaaggtgaaa
tggactgggacaactcggctgcaatgcaagtgggaatgcgtgtggtgagaggtatagactggaaatggtcaaaccaggacaatggtgaaggcagcatggg
cactgtggtagagatcggacgtcagggaagccctaccacacctgacaaaacagtagttgttcaatgggatcatggcaccaggactaattatcggactggc
ttccaaggagcttatgaccttctgctgtatgacaatgctcagacaggtgtccgccatcccaacatcatctgtgactgctgcaagaagcatggaatccgtg
gcatgcgctggaagtgcaaagtctgtttcgactatgatctgtgcacacagtgctatatgaataacaagcacgatttgtcccatatcttcgagagatatga
gaccgccgattcccgaccagtcatactgagcactcggcaaggcttgccccgggttgtgttaaaaggaatcttccaaggtgcaaaagtggtgcgtggtcct
gactgggaatggggaaaccaagatggtggggaaggtaaagttggccgagtcgttgatatccgtggatgggatgtagagactggccgcagtgtggctagtg
taacatgggctgatggaaccactaatgtgtacagagttggccacaagggaaaggttgatcttaagtgcattactgatgctccgggaggtcattattacag
ggatcacttgcccaagcttggtaaacctgctgagttgcaaagaaaagaaagttcagaaaggcacccgtttcagcatggagacaaagtgaagtgtctttta
gatgttgagatattacgagagatgcaagaaggacatgggggatggaaccctaaaatggcagagtttataggtcagactggaacagtacataggattacag
agcgaggtgatgtcagagttcagtacaacagtgagacacgatggacatttcatcccggggctctaacaaagcaaaacagcttttgggttggtgatgttgt
gcgtgtgatggataacatggatgcagtcaagaagctccaagttggtcatggggagtggacagatgatatggtttctgcgctgggtcaaattggcaaggtg
atcaaagtttttggggatggagacatgcgagtttctgttggaggccagtcctggacattcaaccctgcgtgcctcacgtcataccaacgggaagaggatg
ctaacctcatgaccactgaaaatgccaaggagtctaaaaacaccctggtttccatattagaaaagttgctttctcagaagactgattgtgaaagtcctac
ttccttggttattgaagcagcccagggcaatacagccaaagtcagggagatgctccagaaataccccgacaaggtggatattaagaatcagggcaggacg
gctttgcaggttgcctcccatttaggctatatggaggtggtgaaggttttgcttcaagccaatgcaaacattgacctcagggatgacgagggggacactg
ctttgcattatgctgcttatggaaaccaggctggtgtggttagagttttgctatctaaaggagccaatgcagaacttctaaacaatgccaagtgtacagc
gctctacattgcagttaacaaagggttcacagaagtagtccaagtcctctgcaacccaaattgtgccatcaacatgcaggattcctttggggacacacct
ctgcattatgcaatcaccgcagactttagaagcatcattgagatcctcactgaagtacccaatatagatttcactgtgcagaacaatcaagggtttaatc
tactgcatcactcagctctcaagggaaatgtcctggctgtaagcaagatcctggagagagcaagacagcttgtggactctaaaaaagaagatggtttcac
tgcccttcatcttgcaacactgaacaaccaccaggaggtggtagaaatattgatcaaagagggccgctgtgatgtcaatctcaggaataacagaaaccag
acccctctgcatttggctgtcgcccagggccacatcagcttggtgcatctcttagtgactgagggtgccgatgtgaatgcagaggatgaggatggagata
ccccaatgcacatagtatttgtgcgacagcatctcaaatccattgactctcagcaagaaggcaatgggtcatcactactcacaaagttggaggcctctgg
gttgccgggaaatatagagttaaatgttggttcagccatggcttgctttttagctctgtctggagctgacattaactatgccaatcaccgtgggaagtct
cccctggacctgatcgcagatggaaggataacgcaccttgtgaaaaactttgcacagaaattcagagaacaagtggagttagactcaccaaccgtcagtt
gcagcctccgcagagtccacactacacctaatactatgactaaccttagcccctgtagtgccgccagtcctgcagagtgtctagtatgctctgagctggc
cgtactagtcagtttcttcccctgccaacacagcatagtatgtgaagaatgttccagacgaatgaagaagtgcatcaaatgtcaagtgtgcatagcaaga
aagcttaggcaagacagtactgaagttgaatgcagtcccagtttagaagtgtcagaccagaagaaagtaatggaggagctccagaatcgataccgccaaa
tggaggaaaggatcacctgtcctatttgtatcgacaatcacatcaagctggtgttccagtgtggacatgggtcttgtactgaatgcagcacttccctgac
tgcctgtcccatatgcaggcagcttatcagagagaggatacagatatttgtctgagtaggaaagaaagtgcatcttctggatctgagacgtaactgtgga
tttagtagacacaagcaacatgtctgtgccaagcatggctgtgtataccctcttcatggcaagactttctgtatgggaaaaaagggatgactctgcttat
gacgaatatggctgctgtgtgcgtctgacactgtacaaacaaagttctatctgtattatctgtactggaagggctaatcaatactgcagtcttcctgaag
gattttattttaaagagcggcaaataagtttccctaaatcatcttgttactgaaatgaggatttcatctctgccaatgccaccctaagatttctttgctg
gttcttatcttatttgtgtttctgcaatacaacctgatccactgtcaaaaaaaaaaaaaaa
5' EST: CX910777 [+]caagctggaagagcaggcacaactgctctgcccctaaccacaagggatgcgtatctgcctggtgtctgttacgcatgaggaaataagggctaaataaacc
tggctgctttgtacactgtttggacatcaggcctctaagctttctgctctatcagtaaacttcttcaatgcctgcagtagttcagttgctaaggtgaaat
ggactgggacaactcggctgcaatgcaagtgggaatgcgtgtggtgagaggtatagactggaaatggtcaaaccaggacaatggtgaaggcagcatgggc
actgtggtagagatcggacgtcagggaagccctaccacacctgacaaaacagtagttgttcaatgggatcatggcaccaggactaattatcggactggct
tccaaggagcttatgaccttctgctgtatgacaatgctcagacaggtgtccgccatcccaacatcatctgtgactgctgcaagaagcatggaatccgtgg
catgcgctggaagtgcaaagtctgtttcgactatgatctgtgcacacagtgctatatgaataacaagcacgatttgtcccatatctt
3' EST: CX910776 [+]tttttttttttttttgacagtggatcaggttgtattgcagaaacacaaataagataagaaccagcaaagaaatcttagggtggcattggcagagatgaaa
tcctcatttcagtaacaagatgatttagggaaacttatttgccgctctttaaaataaaatccttcaggaagactgcagtattgattagcccttccagtac
agataatacagatagaactttgtttgtacagtgtcagacgcacacagcagccatattcgtcataagcagagtcatcccttttttcccatacagaaagtct
tgccatgaagagggtatacacagccatgcttggcacagacatgttgcttgtgtctactaaatccacagttacgtctcagatccagaagatgcactttctt
tcctactcagacaaatatctgtatcctctctctgataagctgcctgcatatgggacaggcagtcagggaagtgctgcattcagtacaagacccatgtcca
cactggaacaccagcttgatgtgattgtcgatacaaataggacaggtgatcctttcctccatttggcggtatcgattctggagctcctccattactttct
tctggtctgacacttctaaactgggactgcattcaacttcagtactgtcttgcctaagc
Library:
Description: PolyA+ RNA was primed with oligo-dT primer 5'-GACTAGTTCTAGATCGCGAG CGGCCGCCCTTTTTTTTTTTTTTT-3'. cDNA was ligated to SalI adapter (5'-TCGACCCACGCGTCCG-3' and 5'-CGGACGCGTGGG-3'), digested with NotI, size fractionated in 1.1% agarose gel electrophoresis and ligated into SalI and NotI digested pCMV-SPORT6 vector. Library contains the 1.5-2 kb size fraction (additional size fractions contained in the NIH_XGC_tropTe3 and Te5 libraries). Library constructed at the DOE Joint Genome Institute.| Anatomy: testis | Stage: frog |
| Normalized: No | Subtraction: No |
| Vector Details | Vector Map |
Name: pCMV-SPORT6
Type: phagemid
5' Restriction Site: SalI
3' Restriction Site: NotI |  |
Usage:
Suppliers:
