XB-CLONE-3256871
Sequence:
Full length sequence: BC158242
[+]
caggatttctggagtctccttaataaagaaaagctgctcgcctcagctggttcttcacaatcctggttaaaagacagtaaaatctctccgtgtcgacgat
ttctggagtctcctgattaaaaaagatcttcatgtccaggatttctggagtctcctgattaaaaaaagctgctcgcctcagctggttcttcacaatcctg
gttaaaagacagtaaaatatgtccgttgtcaggatttctggagtctcctaattaaaaaagatcttcatgtccaggatttctggagtctcctgattaaaga
aaagcagaaagagatgaagcccttaccttgccgaagcaagagaaacagcacttcagccgcttgaacatgttgtttctatgaagacaaaatgaaaaacatc
actttagcatttcccaatacaggtctccttcccttcaagataccaccatgagctccagccacacccagaagaaccgaggggactccactccgctgcctcc
aatcaacgagcgcctggctttcctgcggccgtccagagagctgttagagtattaccgcaagaagatcgctgagtttgatgaggaacacgaggatctggta
aagcaactggagcaatacaaagctacctttgaggagcagtgtcctgcctcctgctggtagatactataccccatggtccctgtgtcccacagacgccctg
agagaaaaggatttaatggaacgttcagataaggtggccaagcggctgcaattgatggcgcagagatacgaggctctggagaaaaggcgcaacatggagg
tagagggctataagactgacatcaagctgctgagacagcggttaaaggatgtagagaagcaactattcaaggtgacgctgaacatcgggccagaccaaga
tcttgctattctggatgcagtgcggcagggcaacaaaaaaaaaaaaaaa
5' EST: CX908196
[+]caggatttctggagtctccttaataaagaaaagctgctcgcctcagctggttcttcacaatcctggttaaaagacagtaaaatctctccgtgtcgacgat
ttctggagtctcctgattaaaaaagatcttcatgtccaggatttctggagtctcctgattaaaaaaagctgctcgcctcagctggttcttcacaatcctg
gttaaaagacagtaaaatatgtccgttgtcaggatttctggagtctcctaattaaaaaagatcttcatgtccaggatttctggagtctcctgattaaaga
aaagcagaaagagatgaagcccttaccttgccgaagcaagagaaacagcacttcagccgcttgaacatgttgtttctatgaagacaaaatgaaaaacatc
actttagcatttcccaatacaggtctccttcccttcaagataccaccatgagctccagccacacccagaagaaccgaggggactccactccgctgcctcc
aatcaacgagcgcctggctttcctgcggccgtccagagagctgttagagtattaccgcaagaagatcgctgagtttgatgaggaacacgaggatctggta
aagcaactggagcaatacaaagctacctttgaggagcagtgtcctgcctcctgctggtagatactataccccatggtccctgtgtcccacagacgccctg
agagaaaaggatttaatggaacgttcagataaggtggccaagcggctgcaattgatggcgcagagatacgaggctctggagaaaaggcgcaacatggagg
tagagggctataagactgacatcaagctgctgagacagcgggtaaaggatgtagagaagcaactatttcaggtgacgctgaacatcgg
3' EST: CX908195
[+]gccctgccgcactgcatccagaatagcaagatcttggtctggcccgatgttcagcgtcaccttgaatagttgcttctctacatcctttaaccgctgtctc
agcagcttgatgtcagtcttatagccctctacctccatgttgcgccttttctccagagcctcgtatctctgcgccatcaattgcagccgcttggccacct
tatctgaacgttccattaaatccttttctctcagggcgtctgtgggacacagggaccatggggtatagtatctaccagcaggaggcaggacactgctcct
caaaggtagctttgtattgctccagttgctttaccagatcctcgtgttcctcatcaaactcagcgatcttcttgcggtaatactctaacagctctctgga
cggccgcaggaaagccaggcgctcgttgattggaggcagcggagtggagtcccctcggttcttctgggtgtggctggagctcatggtggtatcttgaagg
gaaggagacctgtattgggaaatgctaaagtgatgtttttcattttgtcttcatagaaacaacatgttcaagcggctgaagtgctgtttctcttgcttcg
gcaaggtaagggcttcatctctttctgcttttctttaatcaggagactccagaaatcctggacatgaagatcttttttaattaggagactccagaaatcc
tgacaacggacatattttactgtcttttaacaggattgtgaa
Library:
Description: PolyA+ RNA was primed with oligo-dT primer 5'-GACTAGTTCTAGATCGCGAG CGGCCGCCCTTTTTTTTTTTTTTT-3'. cDNA was ligated to SalI adapter (5'-TCGACCCACGCGTCCG-3' and 5'-CGGACGCGTGGG-3'), digested with NotI, size fractionated in 1.1% agarose gel electrophoresis and ligated into SalI and NotI digested pCMV-SPORT6 vector. Library contains the 1.5-2 kb size fraction (additional size fractions contained in the NIH_XGC_tropTe3 and Te5 libraries). Library constructed at the DOE Joint Genome Institute.Anatomy: testis | Stage: frog |
Normalized: No | Subtraction: No |
Vector Details | Vector Map |
Name: pCMV-SPORT6
Type: phagemid
5' Restriction Site: SalI
3' Restriction Site: NotI |  |
Usage:
Suppliers: