XB-CLONE-3342280
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Clone Name: IMAGE:7762153
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Gene:
ppm1l
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Species: Xenopus tropicalis
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Sequence:
5' EST: DR884112 
[+]
tagagggctactcgttgcttcccacctagcaccccattagcggaactccccatgctgaaccgttgctgttagtgatagtgaccctttgttttaggtgctg
cagagctgatcccctgggaggagccgacagagagcggggtcacacctgccaagtgccatgcactgatccccctccttccagtccgcaccgccccccctcc
ccggccattccccggcacataaatgatagaggataccatgactttgctgtctctcctgggtaggatcatgcgctacttcttactccgcccggagaccctc
ttcttgctgtgcatcagcctggccctgtggagttacttcttccacacggacgaggtgaagaccatcgtgaagtccagcagggacgccgtgcaaatggtaa
agggcaaggtggcagagatgatgcagaatgagcgcctcggagggctcgatgtgctggaagcggagttctctaagacttgggagtacaagagcaacaatgt
ggccgtgtattccatccagggcaggagggatcacatggaggaccgcttcgagatcatcactgacttggtcaacaagagccacccttccatcttcggcatc
tttgatggccatggaggagagtcagcagctgaatatgtgaaaacccatttgccagaagtgctgaaacaacatcttcaagactttgagagggataaagaga
acagcgtgctatcctatcagaccatcctggaacagcaaattctagccatagaccgggaaatgctggaaaaactctcagtgtcctatgatgaagcaggtac
tacgtgcttaattgctctgttatccgacaaagaactcacagtcgccaacgtaggggattcgcgcggtgtgttgtgtgacaa
3' EST: DR884111 [+]gttttacttgtatttattaagagtgtttgaacctccgttgttgctttttattaaagggacacttgtctgaagcgctgaatattagaaacatataatccca
ttgaacaaacacattttctaaaaacatagaataaatattacttctctagctgatgacacaagcgtggtcttgttaccccccgcataagaataagattaac
acctcagcaggagcgataccctttaatacagataattacttgttaatggaggaataatgttgggtcatacagaatgcacttcctgtgtttcatggctgga
cagttgatgggtacattagctgcatgagtccatgtctaatcctaagacacgggaatggtgctgctgtacatcttaataaggtttccacctcttgctacta
agttaaaataaaattggttgctgcagtccttttgcacgcgtggtgactttcattgttcatctgttttactgctattcttgaactttactaccataacagt
tatgttatcagggcatcctctataaaacgactgtaacacaatgctcttggctccaaagtgaggctcatctaagcgctctttgataaaccgcactgcttct
tcattgctgaaagcatcccatagaccgtcagatgccaagatcataaactctggctgcagtttgtccaggtcaaaagacagaatatcagggtcagagataa
tgacatttagatttttcagaggataatctcccagtgagcgggacatggcaagaatcccttgtaccctccaggagccattgaaactgatgaacccaccagc
tcttttaatcctcttcctttcccttagctggtacggcttatgatcatgggataaggggatagcattcccatctttgtcacacaacaca
Library:
Description: The library was prepared from total RNA by oligo-dT priming (5'- ACTAGTGCGGCCGCCTAGGCCTCGAGTTTTTTTTTTTTTTTTTTV-3') and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and by XhoI digestion, the cDNA was size selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1000 bp were ligated into EcoRI/XhoI-digested pCS107. Average insert size 1364 bp. Reference for library construction: Current Genomics 4, 635-644. Library constructed by Michelle Tabb and Bruce Blumberg (Dept of Developmental and Cell Biology, University of California, Irvine).| Anatomy: testis | Stage: frog |
| Normalized: No | Subtraction: No |
| Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI |  |
Usage:
Suppliers:
