XB-CLONE-3342665
|
Clone Name: IMAGE:7762405
|
Gene:
|
Species: Xenopus tropicalis
|
|
|
|
Sequence:
5' EST: DR883858 
[+]
gcaaggcaatacgttgagggaaaactccagtacagataatatttataagcatatattaaatattgttaaaaatgcttggtgaaatgtactgacatatggt
atttcaaataaaaatcattatttagcctcatatttgttatttctttgttgggagcaaaaccacgaaaaagacatactattttaacaatattatcatggtc
aatacgaaaagttctgacaattagaaaaaatacaaatttttctcattcatccgtggtcaatcttgctttactgatatgcaatctcagacagatctaatca
agctgttaagcagttggacactcccggtcacttgaaaaccaagcaagccttataacatttttttggcctccagaggtcactgttttgctgaaacctatgg
aagagattttactggttgaactgatggataatactacttagctcctacctaccatagagaaccgaaaaaatgcacaataacatgtaaaaaaaaaaaaaaa
caattacattacagaaaaatatatattttttttttttaattaaatttgttattttcctttaaatgacatggagaggctaattcactggaggatgccaaat
gaaaggtagccccagcaaataaaatccagacatcacaatttttcccccctgataaatgctccttttcttgaaaaaaaaaaaaaaagattccatgccacat
ttttactggaatagagtaatattttcccctggtatctttccttctaaaaaaag
3' EST: DR883857 [+]tatatacggtagtcctatcctatggttactggtttgcaaaaacgttatatccatccacttttattcttatgaacccatctgtgcctcatgtattaagagg
ctgtaataaagctgtaacttttagcagtgttagagaattgtgaagaaaaaggctaatacaaatttgcacaaataatatatatgtcttatttacaaagggg
tctttgaaactctcccacctccgtaccagtcacacagtcagtcttccctctcagccactgatgatctgactcctgccaagcagaacattcaacccccagc
aggtatatatgggagagcagtccagcagaatagtcatgctgttgaaaatgaggtaaggacactacttgattgattgtaacaataaacaccttttaatagg
tttttttaaaacttacttcagaacacgtgtaatcatttttatataacaaaaatgtattgttaactgacataaaaacctaattcacgaatatgtttggcac
cccctggaaatccagttggctagatttttttccctaggctggtgctttttttagaaggaaagataccaggggaaaatattactctattccagtaaaaatg
tggcatggaatctttttttttttttttcaagaaaaggagcatttatcaggggggaaaaattgtgatgtctggattttatttgctggggctacctttcatt
tggcatcctccagtgaattagcctctccatgtcatttaaaggaaaataacaaatttaattaaaaaaaaaaaatatatatttttct
Library:
Description: The library was prepared from total RNA by oligo-dT priming (5'- ACTAGTGCGGCCGCCTAGGCCTCGAGTTTTTTTTTTTTTTTTTTV-3') and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and by XhoI digestion, the cDNA was size selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1000 bp were ligated into EcoRI/XhoI-digested pCS107. Average insert size 1364 bp. Reference for library construction: Current Genomics 4, 635-644. Library constructed by Michelle Tabb and Bruce Blumberg (Dept of Developmental and Cell Biology, University of California, Irvine).| Anatomy: testis | Stage: frog |
| Normalized: No | Subtraction: No |
| Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI |  |
Usage:
Suppliers:
