XB-CLONE-3536070
Clone Name: IMAGE:7720744
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Gene:
wdr83
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX995680
[+]
aatgtatcagtagggggctggagaggaacatcagtatgtgagcccaggtcaccgcgtcactgctgccatttagcgtcactatgtcctttccagccccgaa
acccaaagctccagagctaccccagaagctgcagcacaaactcgagtgcaatcagggggctgtgcgggcagtaaggtttaatgtggatggcaattactgc
atgacctgcggcagtgacaagaccctcaagctctggaatccacacaagggaactttgctgaaaacctacagtggccatgggtacgaggtgctggatacgg
cagggtcatacgacaacagccagatgtgctcctgtagctcagataagacagtcatcctgtgggatgtggcccagggtcaggtggtccggaaattccgtgg
acatgcagggaaggtgaattgtgtgcagtttaacgaggaggcaacagtgattatgtcaggctctatagactcctcaataaggtgctgggactgtcgctct
cgtagacccgaagcaattcagattctggatgaagccaaagatgggatttccagtatcaaaatatcagaccacgagatcctggcagggtcagtagatggga
acctgcgtcgctatgaccttcggaagggagagatgtgcgcagattatcttggcagcccaatcacatgtgtgagcttcagtcaggattcacagtgccttct
ggcatcgtccttagactccacattgcgcctgctggacaaagacacgggagagctgctgggagagtacacgggacaccagaaccacagttacaagttggac
agctgtctgagtgagaaggacactcacgtgctgagctgctc
3' EST: CX995679
[+]taagggcgagaggcttgcagatgccttcagcttcccccttcctcttcataagacgcccctctccagagctgcacccgtccttcgctggcagtgagcaggc
agcactcgctgggatggaaggagagcgactgcaccgctgctttaccgacgggaagtttcagaaccaatgagccctccaccaaatcccagaaacacaccgt
cccatcttctgagcagctcagcacgtgagtgtccttctcactcagacagctgtccaacttgtaactgtggttctggtgtcccgtgtactctcccagcagc
tctcccgtgtctttgtccagcaggcgcaatgtggagtctaaggacgatgccagaaggcactgtgaatcctgactgaagctcacacatgtgattgggctgc
caagataatctgcgcacatctctcccttccgaaggtcatagcgacgcaggttcccatctactgaccctgccaggatctcgtggtctgatattttgatact
ggaaatcccatctttggcttcatccagaatctgaattgcttcgggtctacgagagcgacagtcccagcaccttattgaggagtctatagagcctgacata
atcactgttgcctcctcgttaaactgcacacaattcaccttccctgcatgtccacggaatttccggaccacctgaccctgggccacatcccacaggatga
ctgtcttatctgagctacaggagcacatctggctgttgtcgtatgaccctgccgtatccagcacctcgtacccatggccactgtaggttttcagcaaagt
tcccttgtgtggattccagagcttgagggtcttgtcactgccgcaggtcatgcagtaattgccatccaca
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI |  |
Usage:
Suppliers: