XB-CLONE-3540680
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Clone Name: IMAGE:7719061
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Gene:
actr1a
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX981944 
[+]
ctttccaactatgtgggaaggcccaaacatatccgagtcatggctggtgccctggagggagatttgtttatcggaccaaaggcagaggagcacaggggcc
ttctctcaatccgttaccccatggagcatggaatagtaaaggactggaatgacatggaacgtatctggcaatatgtctattcaaaagaccagctgcagac
attctctgaggagcatcctgtgctactgacagaagctcctttgaacccccgtaaaaatcgcgaaagagcagcagaagttttctttgaaacatttaatgtt
ccggcactgtttatctccatgcaggctgtgctgagtctctacgcaacagggaggacaacaggggttgttctggactctggagatggcgtgactcatgcag
ttcccatttatgaaggatttgccatgcctcattcaatcatgaggatcgacattgcaggcagagatgtttcccgtttcctgcgtctttacctccggaaaga
aggctatgatttccacacctcagcggaatttgaaattgtgaaaaccatcaaggagcgagcatgttacttgtcaataaatccccagaaagatgagacactg
gaaactgaaaaggctcaatactacttacccgatggcagcactattgagattggcccagcccgattcagagccccagaattgcttttccgaccagatctga
taggggaggagtgtgagggaatccacgaggtgttagtatttgccattcagaaatcggacatggacctgaggcgaacgctgttttccacatagtattatct
ggcggat
3' EST: CX981943 [+]tgtgtttctcaaaccagttgggcaggacaattttgctttgtagactcaagtcaatgttaattatctagaagccaggaaggcgatttaagtgcaggtcagc
aagtgaccatggctcgagccactgtgcatactctgactacagtgggatccttcatgttctagcttacaggtatgtcaccctgctgcgccaatatgcaaca
ggcagagtacaaaccctgcagcaacataataaacataaataggaatggaataaaggctttcttctccttggatctctgctggcctttataaatatcagat
cttttccccctcatgagaccatttcttggcatgctggagagcgcatacagacacatacatacacacccacccaccacagtcatctcccgtggacactctc
atactctggaaagcgagttttaaacttagtggacagataaagagactgggtctttcgggacgttagaaggttttcctatggatcgccctggctccgtctt
cttcatattccttctttgacacccacattttcttaaaggtatccagtgatgccaaaatagaaccaccaatccaagtggaatataatctctcctgaggagc
tgatattcttattttgacatcttttggtgctaattttttcacttcacttaaaagtctgtctccaaaacctttaaaaagtgtggatccgccagataatact
atgttggaaaacagcgttcgcctcaggtccatgtccgatttctgaatggcaaatactaacacctcgtggattccctcacactcctcccctatcagatctg
gtcggaaaagcaattctggggctctgaatcgggctg
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).| Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI |  |
Usage:
Suppliers:
