XB-CLONE-3541214
Clone Name: IMAGE:7718868
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Gene:
sypl1
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX981576
[+]
gttaccgagcgctaaactggaggaaagcactgtctgaacagaatttggagggaagagtaggcgtattgagtatataggcgtgacgcgagcagcagagcga
gttatcgcctgaagaaagaaaatggtcggctggcagctgaaccttggcccgctaaaggagcccctgggctttatcaaattattggaatggtttgcttcaa
tcttttcatttgccacttgtggtggttacagtggcaaaactgaattaatagtaagctgtttaggatcttcagaaaataaaactgctaccgcttcatttat
gtatccgtttcgattaaatgttcaagtgtttgaagggatacccaaattattctgtaatcaaacatggaaagactttcaccttacaggagatttttctgcc
ccagcccagttctacgtttctattggagtctttgcctttctttactgcatggctgctcttgtgttttatctaggttttatgcagttgtatcgggactctc
aaaaattcccattatgtgacttcttcattacggtcttctttgctttaatgtggctggttagttcctcagcttgggctaaaggtttgacagatataaaagc
ctccaccagccctcaaggtattgcacagtatacctgcccgactggttacaaatgtctgcctgggcaagagtcacacatgggaagtctgaacatctctgtg
gcgtttggatttttgaatctgatactgtgggcaggaaatgcttggtttgtatacaaggagacaagcctacatactcctccacaacaaccaaacccggagc
agcacctggaaatgtttctccatgaatttaaagataaatcctatagaaaatgcaattcacatttaatggcctacaatc
3' EST: CX981575
[+]ctgattcagaacaataattcactatacatggagaaaaacagttaggcatataaacagtattgggaaatagggtaaatgtgtgtctagaatgcaaacttgt
ggcaaaagctacaatagggctgtaattgcttgtacatattcagtaacctgcacaaccatctatttccattaacattttcaacagtgctctaaaaaatagt
acatgtaaaagagacgaacttggggttttgtgcaataaaataagagaaacaaatgttcaaaatatctagaatataaaaaaaagcatttatactggtaagc
ttttatattaaacttaagcattgcagagagtttgcctattatatgacaaaagcttttggtttaagaggaaatcccaatttttatagttacatcagaatag
gtattccaaattagtacagtacttcatattctgtcatttttcaaaacaacatattgaagaaatatttatcagtgtttgcactcctaaaaaccttagtgtt
ttaaagccaataaactgcagtacatcactgttcacaacaaaacattgttctgtaactggaaaacctgcttgaaataagtcgtaggcctgtttttcagaaa
gaggaatcctggcatgaataccttgtatccacatgtttattaacacataaacaatgcaatccttggcattgaaaacaccagattgtaggccattaaatgt
gaattggcatttctataggatttatctttaaattcatggagaaacatttccaggtgcttgctccgggtttggttgttgtggaggagtatgtaggcttgtc
tccttgtatacnaaccaagcatttcctgcccacagtatcagaatcaaaaatccaaa
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI |  |
Usage:
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