XB-CLONE-3548204
Clone Name: IMAGE:7716052
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Gene:
snx6
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX976814
[+]
ccaagggccattaatgttgatctgcagccagatgcaacccttcaagttgatatttcagatgcactgagcgagagggacaaagtcaaatttacggttcata
ccaagagctcattaccaaacttcaagcagaacgagttttccgtagtcaggcaacatgaagagtttatctggctccatgattcctttgtagagaatgagga
atatgctgggtatattattcctcctgccccgccaaggccagattttgatgcttccagggagaaactacagaaacttggggaaggggaaggatcaatgacc
aaggaggagtttaccaaaatgaagcaggaacttgaagcagagtacctggctatctttaagaaaacagttgcaatgcatgaagtcttcctgtgccgagttg
cagctcatcccattcttaggaaagatctcaacttccatgtctttttggagtacaatcaagacttaagtgtccgaggaaagaacaaaaaagaaaagttaga
agacttcttcaagagcgtggttaagtcagctgatggagtcatcgtttctggcaccaaggatgtggatgatttctttgaccacgagcgaactttccttgtt
gagtaccacaatcgagtaaaggatgcaagtgccaaatcagataagatgacacgatcccataaaaatgcagcagatgactacaacagaataggctcatcaa
tgtacaccctggggacacaggacaactcagatatatgcaaatttttcttgagagtctcagagttattcgacaaaactcgtnaaattgaagctcgagtggc
agctgatgaagacctaaagctttcggacctccttaaatactatttaagagaatcacaagctgctaaagatcttctgta
3' EST: CX976813
[+]cgagaggcttttaagtctacaagttaaggcaaacatattgatgcattagcgttaggaacaaatgtaaaaagataattggagcgctctgagatatctaaga
aatgtctgataaaaaaaatatagagtccggtaaatcaaggtaacagaggcttaaaaaaatttattacatggaacctggggtaagatatacgacacgtacg
actaatgacaaatattaaacccattcgaaccgtgaatatctaacatcatccaagattaacatatattggtaaataagattggcagcgttatgtaatacaa
ttcataacaagaggagtatttatcttcacaaggagaaatgatgaatcatattgtgaaggacagaaagcatacaatgagtaaatcaggaagtgtcagcttg
tggctcactgtagaactacaactcgcaacatccaatactgcagttcagctgggatgccaaggctgaccctccctggactaaataaaggtacacatggata
catagagcgggcctacaagcacttggctggttcttatttggaggctgcccattttctgtgcatgagatcatcctctgccgagctgggaacgccgatgacg
ttcatccaaggaaagtgataactctaggatgtggaaggagggtctattattatggaaataaggacggagtgtgctttatggtctgccgtttaacactgcc
aaacagctttgcaggagctgtaagttacccttggcatgctttagttccagttcagccagttctacaaggttcttgcgaaatgctgccactctccgggttt
taaagtccataagttcttgctttgcagattctgatatcttctcaaacttctgacagctcaactgctgtgtggtttccgctgctanaacatccttggttct
ttgctcttgctttatctagtgctt
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI |  |
Usage:
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