XB-CLONE-3549736
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Clone Name: IMAGE:7715755
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Gene:
lgals4
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX975725 
[+]
catcgattcgtttaggactcctattcatggcggcctccgtgctgggatgtctgtctacattcaggggactatccccaaccacattaccaggtttgctgtg
aatttcacttgtggccaatttgatggatcagacattgcctgccactttaatcctcgctttgatggcaaagacaaggttgtgtttaacagtcttattgggg
gctcctgggggtccgaggagaaaaagaaggattcctttccttttcgcaaagggaagagctttgacctcgcattcatgatcaaccaaagcgcctttgagat
cacagtgaatggctcttccttctataagttcaaacatcgtatgccactggagcgtgtgaacagtttgcaagtcagtggggatgtgactgttcagtccctg
accattgctggaggtggtggtggaatgccagcggcccctatgcctgcacccagtttcccacatccggggggggtgatgccattaccagttttcccagctg
gcaacctacctgtaatgggaggacctgtgtataacccaccagtaccctattatggctctatccagggaggcatgtcaccgagaaagacagtgataattag
agggtttattccagaaggtgcagaaaggttccatataaactttaaggcaggatcttccaacgatgttgctctgcattttaaccctcggttgactgaaaat
gtagtggtgagaaacagtcgacttggaggatcctggggacaggaagaaaggaacctctcgtacaatccattcagacctggacaatattttgatatttcca
tacgctgtgggatggatcgcttcacagtttttgtgaatgggcagcatttctgtgactttgcacacagatattcccatg
3' EST: CX975724 [+]gttacttcagaatttattttggtactgaatgacattttttctgtggcatgtctgggggcaaatcagatctgcacgagagacaaaaccacatttccctcaa
cttctatcctgtcaagcatctggaacatggaatatctgtgtgcaaagtcacagaaatgctgcccattcacaaaaactgtgaagcgatccatcccacagcg
tatggaaatatcaaaatattgtccaggtctgaatggattgtacgagaggttcctttcttcctgtccccaggatcctccaagtcgactgtttctcaccact
acattttcagtcaaccgagggttaaaatgcagagcaacatcgttggaagatcctgccttaaagtttatatggaacctttctgcaccttctggaataaacc
ctctaattatcactgtctttctcggtgacatgcctccctggatagagccataatagggtactggtgggttatacacaggtcctcccattacaggtaggtt
gccagctgggaaaactggtaatggcatcaccccccccggatgtgggaaactgggtgcaggcataggggccgctggcattccaccaccacctccagcaatg
gtcagggactgaacagtcacatccccactgacttgcaaactgttcacacgctccagtggcatacgatgtttgaacttatagaaggaagagccattcactg
tgatctcaaaggcgctttggttgatcatgaatgcgaggtcaaagctcttccctttgcgaaaaggaaggaatccttctttttctcctcggacccccaggag
cccccaataagactgttaaaacacaccttgtctttg
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).| Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI |  |
Usage:
Suppliers:
