XB-CLONE-3555152
|
Clone Name: IMAGE:7713656
|
Gene:
drg2
|
Species: Xenopus tropicalis
|
|
|
|
Sequence:
5' EST: CX971993 
[+]
cggagatcgaaagggagatcgccaggacccagaagaataaggccactgaataccatctgggcctgctaaaggccaaacttgcaaagtaccgagctcagct
cctggaaccatccaaatctgcagccaacaaaggagagggctttgatgttatgaaatctggagatgcccgggtggctctgattggctttccatctgtgggc
aagtctaccttcttgagcttgatgacatccacagctagtgaagctgcatcctacgagtttactacactcacttgcatcccaggagtgatcgagtacaagg
gggccaatattcaacttcttgatctcccaggaattattgaaggagcagctcaggggaaaggaagaggtcggcaggtcattgcagtggcacggacatcaga
cgtggttataatgatgttggatgccacaaaaggagaggttcaaagatcattactggagaaagagttggaatcagtgggcattcgtctaaacaaagacaaa
cctaacatatacttcaagccgaagaaaggaggcggaatctcatttaactccacagtacccctaacccagtgttcggagaagctggtgcagcttattctgc
atgaatacaaaatgtttaatgcagaggtgctgttccgagaggattgcagccctgatgatttcatagatgttatcgttggaaacagagtttacatgccatg
tctctatgtatataacaaaattgatcagatttccatggaggaggtagaccgactagccagacagcctaatagtgtggtaatcagctgtgggatgaagcta
aatttggattatttactggagatgctctgggaataccttgcacttacctgtatttacacaaagaaaagaggagagagac
3' EST: CX971992 [+]ccagtctgaatccatttatttagtgctgttcagtaaatcctatggaagtccatggaaaagaatattccccccagtcactttttaataattggtggctatt
ttcacgatttttttcacactactaaattcttcccacagtcttcaagagctaccattgaccttaaccctttctgcaaagagatggcaagaaactgggggaa
atggcttcttctctgaagatgaaagcatgtgatatgcttaaagcaatttatttcttgacgacctggataacatcttcatgttccatattgtgggtcaggc
ctactctctggggactgtatttggtgctggtgccccaaaccagggcatatttaaactggctggcaagtgttctgtgtattcgatgacacacatgctccac
tgaagctcctttccgtaggatgattgcatcagcaaaatctggtctctctcctcttttctttgtgtaaatacaggtaagtgcaaggtattcccagagcatc
tccagtaaataatccaaatttagcttcatcccacagctgattaccacactattaggctgtctggctagtcggtctacctcctccatggaaatctgatcaa
ttttgttatatacatagagacatggcatgtaaactctgtttccaacgataacatctatgaaatcatcagggctgcaatcctctcggaacagcacctctgc
attaaacattttgtattcatgcagaataagctgcaccagcttctccgaacactgggttaggggtactgtggagttaaatgagattccgcctcctttcttc
ggcttgaagtatatgttaggtttgtctttgtttagacgaatgcccactgattccaactcttt
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).| Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI |  |
Usage:
Suppliers:
