XB-CLONE-3555757
Clone Name: IMAGE:7713361
|
Gene:
ndufs2
|
Species: Xenopus tropicalis
|
|
|
Sequence:
5' EST: CX971582
[+]
cacgtggcgcctacgccggatggaagaaccacacccatcacatggtcatataagagctttgccggttcccagccaatcctgtcatgctgttctttggtgt
gagccaatcagattatcgatccacttccccaaacacaatatcctgagttccaataatagcaacaacatcggcgagcatgtgtccttgtgacatcttatcc
aaacctgccaggtgagcgaatcctggggctttaatcttacagcggtacgggcgactgctcccatcggataccaggtagaccccaaattcacccttaggtg
cctcaatagcagtgtaagtggcccctgggggcacctggtatccttcggtgtaaagtttgaaatgatggatcagactctccatggagcgcttcatttctga
tctcttcgggggagacaccttggcatcatctacttttatctctccttctggcattttgttcagacactgaaggatgatccgcattgactggcgcatttcc
tccacacgacacaggtatctgtcataacagtctccccgtgaaccaattgggacatcaaattcaacctgatcatacacatcatagggctgtgatttcctca
agtcccactggatcccagagccacgaagcatgacaccgctgaatccataattcaaagcatcctcagctgaaaccactccaatatccacagttctgttctt
ccaaattctgttgtttgtcaacatctgcaagcacaggattatgggtaaggaaagctgcctaattcgaaggcacaagggagccgcaccaaactactggcag
tctcatttagagttccattc
3' EST: CX971581
[+]aagcactcaccccaaatccccccctaactggccttcaggctgggcccccttagcccataacaaggttacagatatatagaaacattggggtaacagtcac
actggagcaaacatttatatcacttaaactgcacactgcctcttacatagggttgaaaaaagaccagtgtccatcaagttcaacccatccaagtaaaccc
agcacacctaacccacacctaccaatctatacactcacatacataaactataaatacaaccagtagtactaactgtagatattagtatcacaatagcctt
ggatattctgcttgttcaaaaactcacccaggcccctcttataggcattaacagaatctgccattaccccatcactaggaagggcattccccaaccccct
cactgccctcaccgtgaaaaaccccctacgctgcttcaaatggaagctccgttcctctaatctataggggtgacctctggtgcgttgattgtttttatgg
gaaaaaagaacatccccatctgcctataatcccctctaatgtacttgtacagagtaatcatgtcccctcttactcatactgactcaaaaagaagcagaac
actgcatttgatgtcaggctttatcatttatttatatagccccaggtagttacacagcactttacgttaatgtttgaagaataggggccttacagtaatg
taacaaacaagggcagagagtataacatgngatcagagggagtattgaagcttctgatgctataggcctgggattctaactgtttgagaatggaactcta
aatgag
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI |  |
Usage:
Suppliers: