XB-CLONE-3558980
Clone Name: IMAGE:7712237
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Gene:
lgals4
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX969335
[+]
tcatggcggcctccgtgctgggatgtctgtctacattcaggggactatccccaaccacattaccaggtttgctgtgaatttcacttgtggccaatttgat
ggatcagacattgcctgccactttaatcctcgctttgatggcaaagacaaggttgtgtttaacagtcttattgggggctcctgggggtccgaggagaaaa
agaaggattcctttccttttcgcaaagggaagagctttgacctcgcattcatgatcaaccaaagcgcctttgagatcacagtgaacggctcttccttcta
taagttcaaacatcgtatgccactggagcgtgtgaacagtttgcaagtcagtggggatgtgactgttcagtccctgaccattgctggaggtggtggtgga
atgccagcggcccctatgcctgcacccagtttcccacatccggggggggtgatgccattaccagttttcccagctggcaacctacctgtaatgggaggac
ctgtgtataacccaccagtaccctattatggctctatccagggaggcatgtcaccgagaaagacagtgataattagagggtttattccagaaggtgcaga
aaggttccatataaactttaaggcaggatcttccaacgatgttgctctgcattttaaccctcggttgactgaaaatgtagtggtgagaaacagtcgactt
ggaggatcctggggacgggaagaaaggaacctctcgtacaatccattcagacctggacaatattttgatatttccatacgctgtgggatggatcgcttca
cagtttttgtgaatgggcagcatttctgtgactttgcacacagatattccatgttccagatgcttgacagg
3' EST: CX969334
[+]gggagaggtttttctgtggctgtctgggggcaaatcagatctgcacgagagacaaaaccacatttccctcaacttctatcctgtcaagcatctggaacat
ggaatatctgtgtgcaaagtcacagaaatgctgcccattcacaaaaactgtgaagcgatccatcccacagcgtatggaaatatcaaaatattgtccaggt
ctgaatggattgtacgagaggttcctttcttcccgtccccaggatcctccaagtcgactgtttctcaccactacattttcagtcaaccgagggttaaaat
gcagagcaacatcgttggaagatcctgccttaaagtttatatggaacctttctgcaccttctggaataaaccctctaattatcactgtctttctcggtga
catgcctccctggatagagccataatagggtactggtgggttatacacaggtcctcccattacaggtaggttgccagctgggaaaactggtaatggcatc
accccccccggatgtgggaaactgggtgcaggcataggggccgctggcattccaccaccacctccagcaatggtcagggactgaacagtcacatccccac
tgacttgcaaactgttcacacgctccagtggcatacgatgtttgaacttatagaaggaagagccgttcactgtgatctcaaaggcgctttggttgatcat
gaatgcgaggtcaaagctcttccctttgcgaaaaggaaaggaatccttctttttctcctcggacccccaggagcccccaataagactgttaaacacaacc
ttgtctttgccatcaaagcgaggattaaagtggcaggcaatgtctgatccatcaaattggccacaagt
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI |  |
Usage:
Suppliers: