XB-CLONE-3561916
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Clone Name: IMAGE:7711181
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Gene:
xnf7
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX967290 
[+]
gatagtgaagacccggtacaacaagcaagctggaagggaggagtattatgtgcattacgtggggttgaaccgccgccaaaacgaatgggtggacaaatct
cgcctggtattgaccaaaccgccaaaggagggtgaaaccaacggcacagaccaagaggagaccaatagcacagagcagccggactctaagactccccaga
agaggaaactcgaggaacccgagcccgaaccgaagaaagcgaaggtggaagaaaaagacgcctctaaaaacgcgtccactctgggagctgccggggactt
tgcggaagagctgacgtgcccgctgtgcgtggagctgttcaaggatccggtgatggtggcctgcggtcacaacttctgccgcagctgcatcgacaaggcg
tgggaagggcagagttcctttgcttgccccgaatgcaaggaatccatcaccgaccgcaagtacaccattaaccgggttctggccaacctggtgaagaagg
ccgtgtgtgcgacgccggtaacgcccgtggagaagaagacgcagcccctagagaaatgctctgagcacgacgaacgccttaagctgtactgtaaggacga
cggcacattgggctgtgtcatctgccgcgattcgctcaaacacgccagccataacttcctgcccatcctggacgctgtcggtgtgtacagggaagagctt
tctgccatcgttgcgcctctagaagcctcccttaaagtgacggagcagctgtctagcgagcagagtgagaagattgatcaacacaataaaaacatgtccc
agtacaaggagcacatcacgagtgagtttgagaagc
3' EST: CX967289 [+]ttttttttttttacttttcagcctctttatttccagaagtaataagcaaatgcaactacattcagagtaatgacaaaatatacaacacaaagattcacat
taaaatgcagaaccggtgaacgaatgacattgcaactaaaactggcggcggttctgccaggttacacgtgaccccttgtcggaactcacaggggttcaac
acaacactggaggttaagtggttaaaaagaaattgtggttctttaaacgttgaatttgatagaaatgtgaaagaaaaacttaagtggggaaggcttggcc
ttcagcaacactaaatgggcggcaggcccgcgtcagggcgcgagaggaggaggaatgtttctaagcgggggaggggcagggcgggtctaattgtgaacga
agcgcagggcatcgacattcttgcccccgtcgtgcaggaagggagagaggtacgggtagagtttctccgtgaatgtagcgctgaacgtgtagatggaggt
catgtcgtcggcattatagaaggagatctgccctccctcgtaatccacgtagacgccaatcttacgggggtgggaggccaaggtcagagccttggatggc
gactccagcgctttgtaggcgttgccgttcctcagccagattgcccagtaaccgttctttgggttcagcttgatcttgcccttgcggttgctcgattcgc
tggccatccccacgtcccaggccgtcttgtcccccacttccacttcccagtaatggcgccccgactcgaagcccctggagcccagcaccaggatacactg
gctgaagcgtttggcgttgtccggcaggg
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).| Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI |  |
Usage:
Suppliers:
