XB-CLONE-3562782
|
Clone Name: IMAGE:7710861
|
Gene:
pmpcb
|
Species: Xenopus tropicalis
|
|
|
|
Sequence:
5' EST: CX966705 
[+]
tgacttgataatttatattaacagtagacatgttattaaaatatatagtaccagtaatccattctgaagagttcacttgctgccatagtagggtgctttg
tttccaagtcttcagtcatctttaacaaatgcacaggatactggctcctgtaattaagccttttgcccctttgaggacatgttaaatgggtctctaggac
aaggctatgcggtacagtgtacacagtcttagtatataacaagtcagaacctttttggcacccgcatctatctagctaagtgcaacaaaagtctggccta
gtgctgcaggtgttatactgagatcccctcatatttattgtcttagccagtacattccacttcgtattctgtcataatcaggaagttgtccaataggacc
gacagcagcaactgcagggctcttgttataaatgtatttggtgcatacttcacgaattgtttcagcactgatcaaatcaattcttgcttcaagttcaggc
agtggaattctgcggttgtaacataacatctgcctaccaatatcctcacagataggtgttgacccgtccagctgaagcaacatgtttgtttttagcagat
tcttagcacgcgccacctcattttcagtcacactagtacagagtcggatccattctctctgaacaaaatgcatcatatcttccaccgtgttgggttcaca
caccatatagagcccccagagtccagtgtctgtgtaacatgtgttgaacgactganagctgtgacagaggtttccatggcatgtgagctgggctagttta
ctggataaattcacaccaccgccaaatgatctgtcccagtttccaataagtgtattggcaaccatg
3' EST: CX966704 [+]cagactgtgtactatgcaaaagctttttccaagatctgccgagagcagtggaaatccttgcagacattattcagaacagcactctaggtgaggctgagat
tgaacgggaaagaggagtcatcttacgggagatgcaggaagtggagaccaatctacaagaagtggttttcgattatctgcatgcaacagcttaccacaac
actgccctgggaaggacaatccttggacccacagaaaatatcaaatccataaatcgcaatgatttggtggagtatataacaacacattacaaagggccta
gaatagttctggcagctgcaggaggtgtatcacatgatgaattgctccacttggcaaagtttcactttggtaatttaccatccacgtatgaaggggaaac
ccttcctccgtgctccttcacaggtagtgagatccgagttcgagatgataaaatgccacttgcacacattgcagttgccgtagaagcagtgggttggtct
catccagacacaatcccactcatggttgccaatacacttattggaaactgggacagatcatttggcggtggtgtgaatttatccagtaaactagcccagc
tcacatgccatggaaacctctgtcacagctttcagtcgttcaacacatgttacacagacactggactctgggggctctatatggtgtgtgaacccaacac
ggtggaagatatgatgcattttgttcagagagaatggatccgactctgtactagtgtgactganaatgaggtggcgcgtgctaagaatctgctanaaaca
aacatgttgcttcagctggac
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).| Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI |  |
Usage:
Suppliers:
