XB-CLONE-3564679
Clone Name: IMAGE:7710057
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Gene:
ndufs2
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX965413
[+]
ataacatgggatcagagggagtattgaagcttctgatgctataggcctgggattctaactgtttgagaatggaactctaaatgagactgccagtagtttg
gtgcggctcccttgtgccttcgaattaggcagctttccttacccataatcctgtgcttgcagatgttgacaaacaacagaatttggaagaacagaactgt
ggatattggagtggtttcagctgaggatgctttgaattatggattcagcggtgtcatgcttcgtggctctgggatccagtgggacttgaggaaatcacag
ccctatgatgtgtatgatcaggttgaatttgatgtcccaattggttcacggggagactgttatgacagatacctgtgtcgtgtggaggaaatgcgccagt
caatgcggatcatccttcagtgtctgaacaaaatgccagaaggagagataaaagtagatgatgccaaggtgtctcccccgaagagatcagaaatgaagcg
ctccatggagagtctgatccatcatttcaaactttacaccgaaggataccaggtgcccccaggggccacttacactgctattgaggcacctaagggtgaa
tttggggtctacctggtatccgatgggagcagtcgcccgtaccgctgtaagattaaagccccaggattcgctcacctggcaggtttggataagatgtcac
aaggacacatgctcgccgatgttgttgctattattggaactcaggatattgtgtttggngaagtggatcgataatctgattggctcacaccaaagaacag
catgacaggattggctggg
3' EST: CX965412
[+]aaacctggcatttggaaaaccctagatatcaagcatgccagattacagattctataaagacgaagacacacacgccgattagtagctgcaactttttaga
aacccacaacgaacccgcgcagcagacagcgcacagacgattagttgcagcaacttatattctaacctgtggtgggaaagttgctgtgatcagtcgctgc
ggccgacttattaaaaagttgtggtgacttatcactgtgtgtgtcttctccgtaactgtatcgtaatgtacagtgttttatttgtacctaatggatatac
aaagaaaccaaaaatatactgtgtttgaataaaacatgcttttatttacaatggtgcataataatatttattattataatattattatggtgtgggtgca
attttaaaattgatgggtgtatcctttttctacctcaagaattatgaccaggttgcagagatctcactgtatttagtctagcccaatttaggaagcacat
tctatgcgcattgataaaagaagaaccaattggttttttttctgctttagttctcagtatgaagcagaactgaaaaaagacagaaataagcacatttcca
gtaatcataaaggatcccctgaaaccaaaacatcgcctatgggagggcttctacaaagaaacagaaagaaaatggtaaatgaggatatacatcagtacag
agaagtggatggtactgagagaccacagagtgctgtcaacgagaaaaatgaatcccagatgagcaattcattatcatcttcatcatctgttgtatctgag
agtgaggattctgttctagaaaccggcatttctcagtgtgccaactgtgccaatccgaaatcagcaccttgct
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI |  |
Usage:
Suppliers: