XB-CLONE-3626438
|
Clone Name: IMAGE:7668140
|
Gene:
ywhah
|
Species: Xenopus tropicalis
|
|
|
|
Sequence:
5' EST: CX855569 
[+]
tgcgtcctgggctgaagtgctgctagtagcagaacctggtccagcgcgctgacagtgttcgggagctagggtggtactggtaccggatccacagcacaga
ccggcgtctgatctagcccgcctgctgaaaggatggctgatcgggagcagctgctgcagagagcccgcttagcggagcaggcggaaagatatgaggacat
ggcggcagcaatgaaatcggtcacagaactgaacgagcctttgtctaacgaggatcggaacttgctgtctgtagcctacaaaaacgtggtcggggcacgc
cgctcctcatggcgtgtgataagcagcattgagcagaagacactagcagatggcaatgaaaagaaactggaaaaggtgaaagcctaccgagagaagatcg
aggcagagctggaggcggtatgtagtgaagtgctgtcacttctagacaagtttctcatcaagaactgcaacgacttccagtacgagagcaaagtattcta
tctaaagatgaagggcgattactatcgctacctgtcagaggtgggctcaggagagagaaagcgaagtgtgaccgaagcatccgaagccgcgtacaaggag
gcctttgaaatcagcaaagaacacatgcagccgacccaccccatccgactgggcctggcccttaacttctctgtcttctattatgagatacagggtaacc
ccgagcaggcttgtctccttgctcagcaggccttcgatgatgccattgctgagctagatacgctaaatgaagattcctataaggact
3' EST: CX855568 [+]agattttatatcctcaaagcagcatttatttcatccaagtaatcccaagacatttaaattgggtgtctggcattatatcagcatccattgtaacaggcga
gtacacactgtgcaggtgtagccagaccatacaaggactgaccagcacacaactaacaatcaaataatgaggccctcaaaagtagcagaaaaataataaa
aaaataaaataaaacaaaatgaaaaaaagagcatccaaacccacaggagatgcagaattataaacacgccataagttaaaagggcactgagaaacctcac
aaagctcacatgtaaaacgtctattgtaagctaaaaaaaaaaaaaaaaaaaaggatgtggctccctatccctctgagcactggccaatcacagtcttagc
tgtggcggcacctcaccagaaggggaccagggcaatattacatgcacaaggaatgctgcacaaattaaaactaaaccagagtatcttaacggaataaaac
aagaccactttgattcattccagcccgtacttgccctatcaactaggagagtttgggcgcctttcttaaaccgcagcttctcttcaaccccgctcaaggc
gagggaaacctaagaaacagctgatgtgccaacgcaatccagcaatgcccaagaggactccgcaagaacccgttctccccaggcaggcagcagtatgagc
agttgtgccaatgatacggatatgataag
Library:
Description: PolyA+ RNA was primed with oligo-dT primer 5'-GACTAGTTCTAGATCGCGAG CGGCCGCCCTTTTTTTTTTTTTTT-3'. cDNA was ligated to SalI adapter (5'-TCGACCCACGCGTCCG-3' and 5'-CGGACGCGTGGG-3'), digested with NotI, size fractionated in 1.1% agarose gel electrophoresis and ligated into SalI and NotI digested pCMV-SPORT6 vector. Library contains the 2-3 kb size fraction (additional size fractions contained in the NIH_XGC_tropBrn2 and Brn3 libraries). Library constructed at the DOE Joint Genome Institute.| Anatomy: brain | Stage: frog |
| Normalized: No | Subtraction: No |
| Vector Details | Vector Map |
Name: pCMV-SPORT6
Type: phagemid
5' Restriction Site: SalI
3' Restriction Site: NotI |  |
Usage:
Suppliers:
