XB-CLONE-3653838
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Clone Name: IMAGE:7652497
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Gene:
cdh4
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC161139 
[+]
gatcgggcggacagtggcagcgcccgggggcagtggagcctcgtaccggcaccgggacacccagggagatacgagccgggggtggggggacccagaccgg
tagcagcatgactgggggaccggggagcaccccattattcctactggcactgagtggagctttaagcggagcgcggagccgggcattgggggcgtacgat
gggaacgcgtccgtatcccgcgcctgcactccgggcttctccgcggaaggttacaccgcgctcgtgtcgcccaatatcatggaaggacagaaactgctca
aagtgaagttcaccggctgcagttcgggggcgcggggcatttggtaccaaaccagtaacccggattttaaagtgggcgcagatggggcagtgtacacggc
gcgcacggtgcggatcccggccaaggaagccatgtttacggtgtctgcttggggccatgaaaccccggagaagtgggaagcggccgtccgactcctcgtg
gaagagacacccctcaaccaaagccaatatgggactggaatccagagccgacagtcagaacaagagcagtctcagagtagtactctgttaccatggcgac
agcatcacagcgggctcagaagacagaagagggattgggtgatacctcctgttaatgtcccagagaactcgagggggcccttccctgagcacctagtgcg
gattcgctctgacaaagaccgggagagtatgatccgttacagcatcaccggagtcggggcagaccagccccccatggagattttcagcatagaccccatt
tctgggcgtatgtatgtgaccagacccctggaccgggaggagagatcatcgtaccatctcagggcccacgctgtcgatatgaacgggaacaaggtggaga
atcccatcgacctttccatctacgttatcgacatgaacgacaaccgaccagaattctcaagtcccattttcaatgggtcagtggacgaagtttccaaacc
agggacttatgtcatgacggtgacggcgcacgatgccgacgacatcaacacgtccaatgggatcgtcatgtaccgcatcatggcccagaacccgcagagt
ccgtcccaggatatgtttgtgattcacagcgagacgggcgtcatcaacaccgtggcggctggactggaccgagagaaagtccaacagtacaccgtcgtca
ttcaggccactgacatggaagggaatctgaaccacgggctgtccaacaccgccacggccatcatcactgtcgccgatgtcaacgacaaccctccggaatt
caccaacaaaatgttcatcggagaggttccggagaaccgcattgatgtggtggtggccaacctgaccgtcgtagaccgggaccaaccctacacgcccaac
tggaacgcggtttataagatcattagtggggatccggccggtcactttaccatcaagacagatccggtgaccaatgaaggaattgttactgtggcaaagc
ctgttgactatgaaatgaataaagccttcctgctgaacgtcatggtgaccaaccaagcccctctggccagtggcatccaaatgtccctccagtctacggc
agcggtgaccatttctatcaccgacgtgaatgaagcgccttacttccccaaccggaacgagccaatcaggaggctagagggagagtcgcctggaaggctg
ctgaccacgttctcagccgtggacccagatcgttctatgcaacaagctctcagatactcaaagctttccgacccggccaactggctcaccattaacacca
ccaacggtcaggtctttaccacgggcgtattggatcgggagtcttcgtttgtgaaggacgacgtataccaggccaaatttctggctacggataacggcgt
ccctccagctagcggcaccgggaccctactcatccagctcattgatatcaatgataatgcccccgagctgctccccaaagaagcccagatctgcgagcgg
ccgaatggcaaggggatcaacatcaccgccagcgacgtggaccgcaagccgagcgccgacccttttgtctttgaactgcccagcgtgccctccagcatcc
gccgcaactggaccatcagccggataaacagtaactacgcgcggctcagcctgcacatcgggtatttggagtccggcatgtatgaggtccccgtcatcgt
cacggattcgggcaacccccccctctacaacacgtccgtcattaaggtgaaggtttgtccctgtgatgacaatggggactgcacgagcgttggagccgtg
gcagcagctggactgggtacaggagcaattatctctatcctcatctgtatcatcatcttgctgtcgatggttttgctgttcgtgatgtggatgaagagca
gggagacctggggatgatgcgagtgacactccgtcgtctcaacttgaacaaaaaacaaacacattttttaaagcactgtttttacaaatggaaaaaaaat
aatttaaaaaaatacaaaaaaaaaaaatttaaaaaacgtaaaaaaaaaaaaaaa
5' EST: CX815226 [+]tcgggcggacagtggcagcgcccgggggcagtggagcctcgtaccggcaccgggacacccagggagatacgagccgggggtggggggacccagaccggta
gcagcatgactgggggaccggggagcaccccattattcctactggcactgagtggagctttaagcggagcgcggagccgggcattgggggcgtacgatgg
gaacgcgtccgtatcccgcgcctgcactccgggcttctccgcggaaggttacaccgcgctcgtgtcgcccaatatcatggaaggacagaaactgctcaaa
gtgaagttcaccggctgcagttcgggggcgcggggcatttggtaccaaaccagtaacccggattttaaagtgggcgcagatggggcagtgtacacggcgc
gcacggtgcggatcccggccaaggaagccatgtttacggtgtctgcttggggccatgaaaccccggagaagtgggaagcggccgtccgactcctcgtgga
agagacacccctcaaccaaagccaatatgggactggaatccagagccgacagtcagaacaagagcagtctcagagtagtactctgttaccatggcgacag
catcacagcgggctcagaagacagaagagggattgggtgatacctcctgttaatgtcccagagaactcgagggggcccttccctgagcacctagtgcgga
ttcgctctgacaaagaccgggagagtatgatccgttacagcatcaccggagtcggggcagaccagccccccatggagattttcagcatagaccccatttc
tgggcgtatgtatgtgaccagacccctggaccgggaggagagatcatcgtaccatctcagggcccacgctgt
3' EST: CX815225 [+]acgtttttaaattttttttttttgtatttttttaaattattttttttccatttgtaaaaacagtgctttaaaaaatgtgtttgttttttgttcaagttga
gacgacggagtgtcactcgcatcatccccaggtctccctgctcttcatccacatcacgaacagcaaaaccatcgacagcaagatgatgatacagatgagg
atagagataattgctcctgtacccagtccagctgctgccacggctccaacgctcgtgcagtccccattgtcatcacagggacaaaccttcaccttaatga
cggacgtgttgtagaggggggggttgcccgaatccgtgacgatgacggggacctcatacatgccggactccaaatacccgatgtgcaggctgagccgcgc
gtagttactgtttatccggctgatggtccagttgcggcggatgctggagggcacgctgggcagttcaaagacaaaagggtcggcgctcggcttgcggtcc
acgtcgctggcggtgatgttgatccccttgccattcggccgctcgcagatctgggcttctttggggagcagctcgggggcattatcattgatatcaatga
gctggatgagtagggtcccggtgccgctagctggagggacgccgttatccgtagccagaaatttggcctggtatacgtcgtccttcacaaacgaagactc
ccgatccaatacgcccgtggtaaagacctgaccgttggtggtgttaatggtgagccagttggccgggtcgnaaagctttgagtatctgagagcttgttgc
Library:
Description: PolyA+ RNA was primed with oligo-dT primer 5'-GACTAGTTCTAGATCGCGAG CGGCCGCCCTTTTTTTTTTTTTTT-3'. cDNA was ligated to SalI adapter (5'-TCGACCCACGCGTCCG-3' and 5'-CGGACGCGTGGG-3'), digested with NotI, size fractionated in 1.1% agarose gel electrophoresis and ligated into SalI and NotI digested pCMV-SPORT6 vector. Library contains the 1.5-2 kb size fraction (additional size fractions contained in the NIH_XGC_tropBrn2 and Brn4 libraries). Library constructed at the DOE Joint Genome Institute.| Anatomy: brain | Stage: frog |
| Normalized: No | Subtraction: No |
| Vector Details | Vector Map |
Name: pCMV-SPORT6
Type: phagemid
5' Restriction Site: SalI
3' Restriction Site: NotI |  |
Usage:
Suppliers:
