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XB-LIB-173
Library Name: Yamamoto/Hyodo-Miura NIBB/NBRP Xenopus DMZ pCS2p+ cDNA library |
Species: Xenopus laevis |
Number Of Clones: 69183 |
Description: About 1000pieces of Keller explants were dissected at stage 10.5 and cultured in 0.1% BSA/1 x steinberg's solution. Total RNA was isolated by the acid guanidinium thiocyanate-phenolchloroform method from explants at stage 11~15. A cDNA library was made with the ZAP-cDNA Synthesis Kit (Stratagene ) according to manufacturer's instruction with minor modification. 4ug of polyA+RNA was oligo dT primed (XhoI dT primer). After second strand synthesis, EcoRI adaptor was ligated and the cDNA was directionally cloned with EcoRI at the 5' end and XhoI at the 3' end into the pCS2p+ vector. The average insert size was about 2kb. The host bacteria was XL2-Blue (Stratagene ).
| Normalized: No | Subtraction: No |
| Anatomy: Keller explant | Stage: NF stage 10.5 to NF stage 15 |
| Vector Details | Vector Map | Name: pBluescript SK- Type: phagemid 5' Restriction Site: EcoRI 3' Restriction Site: XhoI |  |