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XB-LIB-29
Library Name: NIH_XGC_tropNeu5 |
Species: Xenopus tropicalis |
Number Of Clones: 2464 |
Description: The library was prepared from 5 ug of poly A+ RNA by oligo-dT priming (5'-GAGAGAGAGAGAGCTCT(20)V-3'). After ligation of PstI adapters and XhoI and PstI digestion, the cDNA was size selected by chromatography on Sepharose CL-4B columns and fractions containing cDNAs larger than 500 bp were ligated into PstI/XhoI-digested pCS22+. Reference for library construction: Current Genomics 4, 635-644. Library constructed by Jisong Peng and Bruce Blumberg (Dept of Developmental and Cell Biology, University of California, Irvine).
| Normalized: No | Subtraction: No |
| Anatomy: whole organism | Stage: NF stage 14 to NF stage 18 |
| Vector Details | Vector Map | Name: pCS22+ Type: plasmid 5' Restriction Site: PstI 3' Restriction Site: XhoI |  |