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Summary Genes Attributions
XB-LIB-40

Library Name: NIH_XGC_tropMet6

Species: Xenopus tropicalis

Number Of Clones: 582


Description: PolyA+ RNA was primed with oligo-dT primer 5'-GACTAGTTCTAGATCGCGAG CGGCCGCCCTTTTTTTTTTTTTTT-3'. cDNA was ligated to SalI adapter (5'-TCGACCCACGCGTCCG-3' and 5'-CGGACGCGTGGG-3'), digested with NotI, size fractionated in 1.1% agarose gel electrophoresis and ligated into SalI and NotI digested pCMV-SPORT6 vector. Library constructed at the DOE Joint Genome Institute.

Normalized: No Subtraction: No
Anatomy: whole organism Stage: NF stage 62

Vector DetailsVector Map
Name: pCMV-SPORT6

Type: phagemid

5' Restriction Site: SalI

3' Restriction Site: NotI		Expression Image