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XB-LIB-44
Library Name: NIH_XGC_tropKid1 |
Species: Xenopus tropicalis |
Number Of Clones: 11526 |
Description: The library was prepared from 5 ug of poly A+ RNA by oligo-dT priming (5'-GAGAGAGAGAGAGAGAGAGACTAGTCTCGAGTTTTTTTTTTTTTTTTTT-3') and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and by XhoI digestion, the cDNA was size selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1000 bp were ligated into EcoRI/XhoI-digested pCS107. Reference for library construction: Current Genomics 4, 635-644. Primers for sequencing: t3 or t7 for 3' end, sp6 for 5' end. Library constructed by Bruce Blumberg (Dept of Developmental and Cell Biology, University of California, Irvine). Note: this is a Xenopus Gene Collection library.
| Normalized: No | Subtraction: No |
| Anatomy: mesonephric kidney | Stage: frog |
| Vector Details | Vector Map | Name: pCS107 Type: plasmid 5' Restriction Site: EcoRI 3' Restriction Site: XhoI |  |