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XB-LIB-45
Library Name: NIH_XGC_tropFat1 |
Species: Xenopus tropicalis |
Number Of Clones: 6260 |
Description: The library was prepared from 5 ug of poly A+ RNA by oligo-dT priming (5'- ACTAGTGCGGCCGCCTAGGCCTCGAGTTTTTTTTTTTTTTTTTTV-3') and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and by XhoI digestion, the cDNA was size selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1000 bp were ligated into EcoRI/XhoI-digested pCS107. Average insert size 1905 bp. Reference for library construction: Current Genomics 4, 635-644. Library constructed by Michelle Tabb and Bruce Blumberg (Dept of Developmental and Cell Biology, University of California, Irvine).
| Normalized: No | Subtraction: No |
| Anatomy: fat body | Stage: frog |
| Vector Details | Vector Map | Name: pCS107 Type: plasmid 5' Restriction Site: EcoRI 3' Restriction Site: XhoI |  |