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XB-LIB-89
Library Name: Soares NXEG |
Species: Xenopus laevis |
Number Of Clones: 1957 |
Description: 1st strand was primed with a Not I - oligo(dT) primer[5' TGTTACCAATCTGAAGTGGGAGCGGCCGCGAATCTTTTTTTTTTTTTTTTTTTT 3']; double-stranded cDNA was ligated to EcoRI adaptors 5'-AATTCGGCACGAGG-3' and 5'-CCTCGTGCCG-3' (Pharmacia), digested with NotI and cloned into the NotI and EcoRI sites of the pT7T3D-PacI vector. Library went through one round of normalization, and was constructed in the laboratory of M. Bento Soares (University of Iowa).
| Normalized: No | Subtraction: No |
| Anatomy: egg | Stage: unfertilized egg stage |
| Vector Details | Vector Map | Name: pT7T3D-PacI Type: plasmid 5' Restriction Site: EcoRI 3' Restriction Site: NotI |  |