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XB-LIB-99
Library Name: Xenopus laevis unfertilized egg cDNA library |
Species: Xenopus laevis |
Number Of Clones: 2072 |
Description: cDNA from 2 ug polyA+ mRNA, oligo dT-primed using 5'-ACTAGTGCGGCCGCCTAGGCCTCGAGTTTTTTTTTTTTTTT-3'. 500,000 unamplified cDNAs were mass excised (from lambda-ZAPII) using ExAssist phage. The resulting single-stranded phagemids were used to infect TOP10F'. This library has been used successfully to clone a number of full-length cDNAs ranging in size from 1.4-4.5 kb. There are <0.5% clones with multiple inserts. One should be suspicious of any clone which gives 3 or more bands in an EcoRI/XhoI digest AND has an internal XhoI site. Library constructed and donated by B. Blumberg (University of California, Irvine), also referred to as Xenopus_laevis_unfertilized_egg_cDNA_library.
| Normalized: No | Subtraction: No |
| Anatomy: egg | Stage: unfertilized egg stage |
| Vector Details | Vector Map | Name: pBluescript SK- Type: phagemid 5' Restriction Site: EcoRI 3' Restriction Site: XhoI |  |