Return to search results Title: Retinoic acid induced expression of Hnf1β and Fzd4 is required for pancreas development in Xenopus laevis

Summary

Retinoic acid (RA) is required for pancreas specification in Xenopus and other vertebrates. However, the gene network that is directly induced by RA-signalling in this context remains to be defined. We identified the transcription factor Hnf1β and the Wnt-receptor Fzd4/Fzd4s as direct RA-target genes through RNA-sequencing of in vitro generated pancreatic explants. Functional analyses of Hnf1β and Fzd4/Fzd4s in programmed pancreatic explants and whole embryos revealed their requirement for pancreatic progenitor formation and differentiation. Hnf1β and Fzd4/Fzd4s thus appear to be involved in pre-patterning events of the embryonic endoderm allowing for pancreas formation.

Contributors: Gabriela Salinas-Riester, Tomas Pieler, Maja Gere, Claudia Pommerenke, Thomas Lingner

Experiment Type: VegT/Noggin/Cyp26a1 programmed animal cap explants from Xenopus laevis, untreated and treated with RA/CHX and collected 2 hours after RA addition at the equivalent of gastrula stage 11; two replicates A and B for each condition

Experiment Reagents: cycloheximide, cyp26a1, nog, [-][+]


Article: XB-ART-54918, PubMed

Source: NCBI GEO, Xenbase Download

Sample	View		GSMs	Assay Type
animal cap explant + nog + vegt + cyp26a1 - NF12.5 GSM3082043  GSM3082044  RNA-Seq Xla   [-] [+] Background: Xla.Outbred Manipulations: Manipulation Type Reagent Target Gene Stage Treatment Area mRNA injection cyp26a1 (Xla) cyp26a1 (Xla) NF stage 2 (2-cell) blastomere mRNA injection vegt (Xla) vegt (Xla) NF stage 2 (2-cell) blastomere mRNA injection nog (Xla) nog (Xla) NF stage 2 (2-cell) blastomere explant   NF stage 8 animal cap Conditions: Tissue Assay Stages ChIP Antibody animal cap NF stage 12.5      animal cap explant + nog + vegt + cyp26a1 + Retinoic acid + CHX - NF12.5 DEG GSM3082049  GSM3082050  RNA-Seq Xla   [-] [+] Background: Xla.Outbred Manipulations: Manipulation Type Reagent Target Gene Stage Treatment Area mRNA injection nog (Xla) nog (Xla) NF stage 2 (2-cell) blastomere mRNA injection vegt (Xla) vegt (Xla) NF stage 2 (2-cell) blastomere mRNA injection cyp26a1 (Xla) cyp26a1 (Xla) NF stage 2 (2-cell) blastomere explant   NF stage 8 animal cap chemical Retinoic acid NF stage 11 animal cap chemical cycloheximide NF stage 11 animal cap Conditions: Tissue Assay Stages ChIP Antibody animal cap NF stage 12.5      animal cap explant + nog + vegt + cyp26a1 + Retinoic acid - NF12.5 DEG GSM3082047  GSM3082048  RNA-Seq Xla   [-] [+] Background: Xla.Outbred Manipulations: Manipulation Type Reagent Target Gene Stage Treatment Area mRNA injection nog (Xla) nog (Xla) NF stage 2 (2-cell) blastomere mRNA injection vegt (Xla) vegt (Xla) NF stage 2 (2-cell) blastomere mRNA injection cyp26a1 (Xla) cyp26a1 (Xla) NF stage 2 (2-cell) blastomere explant   NF stage 8 animal cap chemical Retinoic acid NF stage 11 animal cap Conditions: Tissue Assay Stages ChIP Antibody animal cap NF stage 12.5      animal cap explant + vegt + nog + cyp26a1 + CHX - NF12.5 DEG GSM3082045  GSM3082046  RNA-Seq Xla   [-] [+] Background: Xla.Outbred Manipulations: Manipulation Type Reagent Target Gene Stage Treatment Area mRNA injection nog (Xla) nog (Xla) NF stage 2 (2-cell) blastomere mRNA injection cyp26a1 (Xla) cyp26a1 (Xla) NF stage 2 (2-cell) blastomere mRNA injection vegt (Xla) vegt (Xla) NF stage 2 (2-cell) blastomere explant   NF stage 8 animal cap chemical cycloheximide NF stage 11 embryo Conditions: Tissue Assay Stages ChIP Antibody animal cap NF stage 12.5

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