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GEO Series: GSE143795

Title: Specification of embryonic mesoderm by Pinhead signaling

Summary

Among the three embryonic germ layers, mesoderm plays a central role in the establishment of the vertebrate body plan. Mesoderm is specified by secreted signaling proteins from the FGF, Nodal, BMP and Wnt families. No new classes of extracellular mesoderm-inducing factors have been identified in more than two decades. Here we show that the Xenopus pinhead (pnhd) gene encodes a novel secreted protein that activates specific mesodermal markers in presumptive ectoderm. We demonstrate that Pnhd is required for the specification of a subset of embryonic mesoderm in vivo. RNA sequencing revealed that many transcriptional targets of Pnhd are shared with those of the FGF pathway. Pnhd signaling involves active FGF but not Wnt receptors and is accompanied by Erk1 phosphorylation. We propose that Pnhd operates in the marginal zone to specify mesodermal progenitors via an FGF receptor-dependent mechanism.


Contributors: Olga Ossipova, Keiji Itoh, Aurelian Radu, Ezan Jerome, Sergei Sokol

Experiment Type: Gain of function and loss of function study of Pinhead, a novel growth factor. Duplicate samples for control ectoderm explants (Cocaps1, Cocaps2) and Flag-Pinhead-expressing caps (FlagPin1, FlagPin2) at stage 11, gastrula were analyzed for Pinhead gain-of-function by RNAseq. For Pinhead loss-of-function, duplicate samples for control marginal zone explants (Co1, Co2) and Pinhead-depleted (Pinhead SpMo) marginal zone explants (PhSpMo1, PhSpMo2) were studied at st.11 by RNAseq.

Experiment Reagents: pnhd MO4, Tg(Xla.pnhd.L-FLAG).

Phenotypes:
Xla Wt + pnhd-FLAG + animal cap explant + animal cap NF11 (RNA-Seq (RNA))

Article: XB-ART-57304, PubMed

Source: NCBI GEO, Xenbase Download

Samples: (DEG = Differentially Expressed Genes; GSM = GEO Sample Number)
Sample View GSMs Assay Type
animal cap explant - NF11 GSM4274730  GSM4274731  RNA-Seq
Background: Xla.Outbred
Manipulations:
Manipulation Type Reagent Target Gene Stage Treatment Area
explant   NF stage 10 animal cap
Conditions:
Tissue Assay Stages ChIP Antibody
animal cap NF stage 11  
   animal cap explant + pnhd-FLAG - NF11 DEG GSM4274728  GSM4274729  RNA-Seq
Background: Xla.Outbred
Manipulations:
Manipulation Type Reagent Target Gene Stage Treatment Area
mRNA injection Tg(Xla.pnhd.L-FLAG) pnhd (Xla) NF stage 4 (8-cell) animal blastomere
explant   NF stage 10 animal cap
Conditions:
Tissue Assay Stages ChIP Antibody
animal cap NF stage 11  
marginal zone explant - NF10.5 GSM4274726  GSM4274727  RNA-Seq
Background: Xla.Outbred
Manipulations:
Manipulation Type Reagent Target Gene Stage Treatment Area
explant   NF stage 10 marginal zone
Conditions:
Tissue Assay Stages ChIP Antibody
marginal zone NF stage 10.5  
   marginal zone explant + pnhd MO - NF10.5 DEG GSM4274732  GSM4274733  RNA-Seq
Background: Xla.Outbred
Manipulations:
Manipulation Type Reagent Target Gene Stage Treatment Area
morpholino injection pnhd MO4 pnhd (Xla) NF stage 3 (4-cell) blastomere
explant   NF stage 10 marginal zone
Conditions:
Tissue Assay Stages ChIP Antibody
marginal zone NF stage 10.5  
Select All DEG      Select All 
Xtr   
Xla 

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