Return to search results Title: HEB and E2A function as SMAD/FOXH1 cofactors

Summary

Nodal signaling, mediated through SMAD transcription factors, is necessary for pluripotency maintenance and endoderm commitment. We have identified a new motif, termed SMAD Complex Associated (SCA) that is bound by SMAD2/3/4 and FOXH1 in human embryonic stem cells (hESCs) and derived endoderm. We demonstrate that two bHLH proteins - HEB and E2A - bind the SCA motif at regions overlapping SMAD2/3 and FOXH1. Further, we show that HEB and E2A associate with SMAD2/3 and FOXH1, suggesting they form a complex at critical target regions. This association is biologically important, as E2A is critical for mesendoderm specification, gastrulation, and Nodal signal transduction in Xenopus tropicalis embryos. Taken together, E2A is a novel Nodal signaling cofactor that associates with SMAD2/3 and FOXH1 and is necessary for mesendoderm differentiation.

Contributors: Se-Jin Yoon, Andrea Wills, Edward Chuong, Rakhi Gupta, Julie Baker

Experiment Type: ChIP-seq of Smad2/3 and Input in X.tropicalis, stage 10.5 embryo.

Article: XB-ART-43683, PubMed

Source: NCBI GEO, Xenbase Download

Sample	View		GSMs	Assay Type
input WE - NF10.5 GSM746612  ChIP-Seq [-] [+] Background: Xtr.Outbred Conditions: Tissue Assay Stages ChIP Antibody embryo NF stage 10.5      Smad2/3 WE - NF10.5 GSM746611  ChIP-Seq Xtr   [-] [+] Background: Xtr.Outbred Conditions: Tissue Assay Stages ChIP Antibody embryo NF stage 10.5 Smad2 Ab9

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Select All  Xtr    Xla

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