GEO Series: GSE43652
Summary
To analyze the dynamics and diversity of coding and non-coding transcripts during development, both polyadenylated mRNA and ribosomal RNA-depleted total RNA were harvested across six developmental stages and subjected to high throughput sequencing. The maternally loaded transcriptome is highly diverse and consists of both polyadenylated and deadenylated transcripts. Many maternal genes show peak expression in the oocyte and include genes which are known to be the key regulators of events like oocyte maturation and fertilization. Of all the transcripts that increase in abundance between an early blastula and larval stages, about 30% of the embryonic genes are induced by fourfold or more by the late blastula stage and another 35% by late gastrulation. Using a gene model validation and discovery pipeline, we identified novel transcripts, the majority of which show hallmarks of being long non-coding RNAs both in terms of their coding potential and gene structure.
Contributors: Gert Jan Veenstra, Sarita Paranjpe, Ulrike Jacobi, Simon van Heeringen, Gert Veenstra
Experiment Type: Profiles of polyadenylated mRNA (6 stages) and ribosomal RNA-depleted total RNA (3 stages) through early Xenopus tropicalis development
Article: XB-ART-47572, PubMed
Source: NCBI GEO, Xenbase Download
| Sample | View | GSMs | Assay Type | ||
|---|---|---|---|---|---|
|
Select All
Xtr
|
Return to search results