Return to search results Title: Innate Immune Response and Off-Target Mis-splicing Are Common Morpholino-Induced Side Effects in Xenopus

Summary

We report on the implications of genetic KO versus MO-mediated KD of the mesoderm-specifying Brachyury paralogues in the Western clawed frog Xenopus tropicalis. While both KO and KD embryos fail to activate the same core gene regulatory network, resulting in virtually identical morphological defects, embryos injected with control or target MOs also show a systemic GC content dependent immune response and many off-target splicing defects.

Contributors: George Gentsch, George Gentsch, James Smith

Experiment Type: Comparison of poly(A) transcriptome between Brachyury null mutants and morphants over two tailbud stages.

Experiment Reagents: tbxt.2 MO3, tbxt.2 MO4, tbxt MO2, [-][+]


Article: XB-ART-54637, PubMed

Source: NCBI GEO, Xenbase Download

Sample	View		GSMs	Assay Type
WE - NF26 GSM2537311  GSM2537312  GSM2537313  RNA-Seq Xtr   [-] [+] Background: Xtr.NigerianNasco Conditions: Tissue Assay Stages ChIP Antibody embryo NF stage 26 to NF stage 26      WE + hbg1 cMO - NF26 DEG GSM2537314  GSM2537315  GSM2537316  RNA-Seq Xtr   [-] [+] Background: Xtr.Outbred Manipulations: Manipulation Type Reagent Target Gene Stage Treatment Area morpholino injection   hbg1 NF stage 1 zygote Conditions: Tissue Assay Stages ChIP Antibody embryo NF stage 26 to NF stage 26      WE +tbxt MO + tbxt.2 MO - NF26 DEG GSM2537317  GSM2537318  GSM2537319  RNA-Seq Xtr   [-] [+] Background: Xtr.Outbred Manipulations: Manipulation Type Reagent Target Gene Stage Treatment Area morpholino injection tbxt MO2 tbxt NF stage 1 zygote morpholino injection tbxt MO3 tbxt NF stage 1 zygote morpholino injection tbxt.2 MO3 tbxt.2 NF stage 1 zygote morpholino injection tbxt.2 MO4 tbxt.2 NF stage 1 zygote Conditions: Tissue Assay Stages ChIP Antibody embryo NF stage 26 to NF stage 26      WE + tbxt+/+, tbxt.2+/+ - NF26 DEG GSM2537329  GSM2537330  GSM2537331  RNA-Seq Xtr   [-] [+] Background: Xtr.Outbred Conditions: Tissue Assay Stages ChIP Antibody embryo NF stage 26 to NF stage 26      WE + tbxt-/+, tbxt.2-/+ - NF26 DEG GSM2537332  GSM2537333  GSM2537334  RNA-Seq Xtr   [-] [+] Background: Xtr.tbxt,tbxt.2emJcsm Conditions: Tissue Assay Stages ChIP Antibody embryo NF stage 26 to NF stage 26      WE + tbxt-/-, tbxt.2-/- - NF26 DEG GSM2537335  GSM2537336  GSM2537337  RNA-Seq Xtr   [-] [+] Background: Xtr.tbxt,tbxt.2emJcsm Conditions: Tissue Assay Stages ChIP Antibody embryo NF stage 26 to NF stage 26   WE - NF34 GSM2537320  GSM2537321  GSM2537322  RNA-Seq Xtr   [-] [+] Background: Xtr.NigerianNasco Conditions: Tissue Assay Stages ChIP Antibody embryo NF stage 33 and 34 to NF stage 33 and 34      WE + hbg1 cMO - NF34 DEG GSM2537323  GSM2537324  GSM2537325  RNA-Seq Xtr   [-] [+] Background: Xtr.Outbred Manipulations: Manipulation Type Reagent Target Gene Stage Treatment Area morpholino injection   hbg1 NF stage 1 zygote Conditions: Tissue Assay Stages ChIP Antibody embryo NF stage 33 and 34 to NF stage 33 and 34      WE +tbxt MO + tbxt.2 MO - NF34 DEG GSM2537326  GSM2537327  GSM2537328  RNA-Seq Xtr   [-] [+] Background: Xtr.Outbred Manipulations: Manipulation Type Reagent Target Gene Stage Treatment Area morpholino injection tbxt MO2 tbxt NF stage 1 zygote morpholino injection tbxt MO3 tbxt NF stage 1 zygote morpholino injection tbxt.2 MO3 tbxt.2 NF stage 1 zygote morpholino injection tbxt.2 MO4 tbxt.2 NF stage 1 zygote Conditions: Tissue Assay Stages ChIP Antibody embryo NF stage 33 and 34 to NF stage 33 and 34      WE + tbxt+/+, tbxt.2+/+ - NF34 DEG GSM2537338  GSM2537339  GSM2537340  RNA-Seq Xtr   [-] [+] Background: Xtr.Outbred Conditions: Tissue Assay Stages ChIP Antibody embryo NF stage 33 and 34 to NF stage 33 and 34      WE + tbxt-/+, tbxt.2-/+ - NF34 DEG GSM2537341  GSM2537342  GSM2537343  RNA-Seq Xtr   [-] [+] Background: Xtr.tbxt,tbxt.2emJcsm Conditions: Tissue Assay Stages ChIP Antibody embryo NF stage 33 and 34 to NF stage 33 and 34      WE + tbxt-/-, tbxt.2-/- - NF34 DEG GSM2537344  GSM2537345  GSM2537346  RNA-Seq Xtr   [-] [+] Background: Xtr.tbxt,tbxt.2emJcsm Conditions: Tissue Assay Stages ChIP Antibody embryo NF stage 33 and 34 to NF stage 33 and 34

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