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Protein kinase C regulation of cardiac calcium channels expressed in Xenopus oocytes.
Bourinet E
,
Fournier F
,
Lory P
,
Charnet P
,
Nargeot J
.
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L-Type cardiac Ca2+ channels expressed in Xenopus oocyte were studied following rat heart ribonucleic acid, messenger (mRNA) injection. We demonstrate that exogenous Ca2+ channels are sensitive to intracellular regulation by protein kinase C (PKC). This was performed by using two types of PKC activators [phorbol esters and a structural analogue of diacyl-glycerol (DAG)] and a specific peptidic inhibitor. Ca2+ channel modulation resulted in an initial increase of the inward current, without any modification of the voltage-dependent properties, and a second delayed phase, specifically observed with phorbol esters, characterized by a progressive decrease in current amplitude. Concomitantly, a reduction of membrane capacitance, reflecting a reduction of the total membrane surface area, was observed. We suggest that this phenomenon underlies the irreversible decrease of the expressed Ba2+ current via sequestration of Ca2+ channels and/or PKC. We also demonstrate that regulation of cardiac mRNA-directed Ca2+ channels by PKC activators was strictly dependent on intracellular Ca2+ concentration, and was partially additive with cyclic-adenosine-monophosphate-(cAMP) dependent regulation.
Adachi,
Possible involvement of calpain in down-regulation of protein kinase C.
1990, Pubmed
Adachi,
Possible involvement of calpain in down-regulation of protein kinase C.
1990,
Pubmed
Armstrong,
Calcium channel regulation by calcineurin, a Ca2+-activated phosphatase in mammalian brain.
1989,
Pubmed
Belles,
A possible physiological role of the Ca-dependent protease calpain and its inhibitor calpastatin on the Ca current in guinea pig myocytes.
1988,
Pubmed
Biel,
Tissue-specific expression of high-voltage-activated dihydropyridine-sensitive L-type calcium channels.
1991,
Pubmed
Bourinet,
Endogenous Xenopus-oocyte Ca-channels are regulated by protein kinases A and C.
1992,
Pubmed
,
Xenbase
Bristow,
Decreased catecholamine sensitivity and beta-adrenergic-receptor density in failing human hearts.
1982,
Pubmed
Chirgwin,
Isolation of biologically active ribonucleic acid from sources enriched in ribonuclease.
1979,
Pubmed
Dascal,
Activation of protein kinase C alters voltage dependence of a Na+ channel.
1991,
Pubmed
,
Xenbase
Dascal,
Expression and modulation of voltage-gated calcium channels after RNA injection in Xenopus oocytes.
1986,
Pubmed
,
Xenbase
Döşemeci,
Phorbol ester increases calcium current and simulates the effects of angiotensin II on cultured neonatal rat heart myocytes.
1988,
Pubmed
Farago,
Protein kinase C in transmembrane signalling.
1990,
Pubmed
Gauthier-Rouvière,
ras-induced c-fos expression and proliferation in living rat fibroblasts involves C-kinase activation and the serum response element pathway.
1990,
Pubmed
Gusovsky,
Selective effects of activation of protein kinase C isozymes on cyclic AMP accumulation.
1991,
Pubmed
Guthrie,
A synthetic pseudosubstrate peptide of protein kinase C inhibits the phorbol-12,13-dibutyrate effect on permeabilized coronary artery smooth muscle.
1991,
Pubmed
House,
Protein kinase C contains a pseudosubstrate prototope in its regulatory domain.
1987,
Pubmed
Huang,
The mechanism of protein kinase C activation.
1989,
Pubmed
Hullin,
Calcium channel beta subunit heterogeneity: functional expression of cloned cDNA from heart, aorta and brain.
1992,
Pubmed
,
Xenbase
Kameyama,
Tissue extract recovers cardiac calcium channels from 'run-down'.
1988,
Pubmed
Kaneko,
A long-lasting potentiation of calmodulin-mediated chloride channel activity without a mediation of protein kinase C in Xenopus oocytes injected with rat brain mRNA.
1990,
Pubmed
,
Xenbase
Lacerda,
Effects of protein kinase C activators on cardiac Ca2+ channels.
1988,
Pubmed
Laurent,
Involvement of a calcium-phospholipid-dependent protein kinase in the maturation of Xenopus laevis oocytes.
1988,
Pubmed
,
Xenbase
Leatherman,
Effect of phorbol esters on contractile state and calcium flux in cultured chick heart cells.
1987,
Pubmed
Lory,
Characterization of voltage-dependent calcium channels expressed in Xenopus oocytes injected with mRNA from rat heart.
1990,
Pubmed
,
Xenbase
Lory,
Cyclic AMP-dependent modulation of cardiac Ca channels expressed in Xenopus laevis oocytes.
1992,
Pubmed
,
Xenbase
Lotan,
Specific block of calcium channel expression by a fragment of dihydropyridine receptor cDNA.
1989,
Pubmed
,
Xenbase
Mikami,
Primary structure and functional expression of the cardiac dihydropyridine-sensitive calcium channel.
1989,
Pubmed
,
Xenbase
Mond,
Bimodal effect of phorbol ester on B cell activation. Implication for the role of protein kinase C.
1991,
Pubmed
Moorman,
Expression of single calcium channels in Xenopus oocytes after injection of mRNA from rat heart.
1987,
Pubmed
,
Xenbase
Moran,
Modulation of a Shaker potassium A-channel by protein kinase C activation.
1991,
Pubmed
,
Xenbase
Nishizuka,
The molecular heterogeneity of protein kinase C and its implications for cellular regulation.
1988,
Pubmed
Ochi,
Modulation of slow gating process of calcium channels by isoprenaline in guinea-pig ventricular cells.
1990,
Pubmed
Perozo,
Phosphorylation affects voltage gating of the delayed rectifier K+ channel by electrostatic interactions.
1990,
Pubmed
Qu,
Purification and characterization of protein kinase C isozymes from rat heart.
1991,
Pubmed
Rane,
Specific inhibitors of protein kinase C block transmitter-induced modulation of sensory neuron calcium current.
1989,
Pubmed
Romanin,
Calpastatin and nucleotides stabilize cardiac calcium channel activity in excised patches.
1991,
Pubmed
Ruth,
Primary structure of the beta subunit of the DHP-sensitive calcium channel from skeletal muscle.
1989,
Pubmed
Tanabe,
Primary structure of the receptor for calcium channel blockers from skeletal muscle.
,
Pubmed
Trautwein,
Regulation of cardiac L-type calcium current by phosphorylation and G proteins.
1990,
Pubmed
Vasilets,
Activation of protein kinase C by phorbol ester induces downregulation of the Na+/K(+)-ATPase in oocytes of Xenopus laevis.
1990,
Pubmed
,
Xenbase
Yuan,
Tumor-promoting phorbol esters inhibit cardiac functions and induce redistribution of protein kinase C in perfused beating rat heart.
1987,
Pubmed
