Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
EMBO J
1997 Jun 02;1611:3320-31. doi: 10.1093/emboj/16.11.3320.
Show Gene links
Show Anatomy links
Licensing of DNA replication by a multi-protein complex of MCM/P1 proteins in Xenopus eggs.
Kubota Y
,
Mimura S
,
Nishimoto S
,
Masuda T
,
Nojima H
,
Takisawa H
.
???displayArticle.abstract???
In eukaryotes, chromosomal DNA is licensed for a single round of replication in each cell cycle. Xenopus MCM3 protein has been implicated in the licensing of replication in egg extract. We have cloned cDNAs encoding five immunologically distinct proteins associated with Xenopus MCM3 as members of the MCM/P1 family. Six Xenopus MCM proteins formed a physical complex in the egg extract, bound to unreplicated chromatin before the formation of nuclei, and apparently displaced from replicated chromatin. The requirement of six XMCM proteins for the replication activity of the egg extract before nuclear formation suggests that their re-association with replicated chromatin at the end of the mitotic cell cycle is a key step for the licensing of replication.
Blow,
Preventing re-replication of DNA in a single cell cycle: evidence for a replication licensing factor.
1993, Pubmed,
Xenbase
Blow,
Preventing re-replication of DNA in a single cell cycle: evidence for a replication licensing factor.
1993,
Pubmed
,
Xenbase
Blow,
Initiation of DNA replication in nuclei and purified DNA by a cell-free extract of Xenopus eggs.
1986,
Pubmed
,
Xenbase
Blow,
A role for the nuclear envelope in controlling DNA replication within the cell cycle.
1988,
Pubmed
,
Xenbase
Bult,
Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.
1996,
Pubmed
Burkhart,
Interactions of human nuclear proteins P1Mcm3 and P1Cdc46.
1995,
Pubmed
Chong,
The role of MCM/P1 proteins in the licensing of DNA replication.
1996,
Pubmed
Coué,
Chromatin binding, nuclear localization and phosphorylation of Xenopus cdc21 are cell-cycle dependent and associated with the control of initiation of DNA replication.
1996,
Pubmed
,
Xenbase
Hartwell,
Checkpoints: controls that ensure the order of cell cycle events.
1989,
Pubmed
Heichman,
The yeast CDC16 and CDC27 genes restrict DNA replication to once per cell cycle.
1996,
Pubmed
Kearsey,
The role of MCM proteins in the cell cycle control of genome duplication.
1996,
Pubmed
,
Xenbase
Krude,
Human replication proteins hCdc21, hCdc46 and P1Mcm3 bind chromatin uniformly before S-phase and are displaced locally during DNA replication.
1996,
Pubmed
,
Xenbase
Kubota,
Identification of the yeast MCM3-related protein as a component of Xenopus DNA replication licensing factor.
1995,
Pubmed
,
Xenbase
Kubota,
Determination of initiation of DNA replication before and after nuclear formation in Xenopus egg cell free extracts.
1993,
Pubmed
,
Xenbase
Lei,
Physical interactions among Mcm proteins and effects of Mcm dosage on DNA replication in Saccharomyces cerevisiae.
1996,
Pubmed
Leno,
Initiation of DNA replication in nuclei from quiescent cells requires permeabilization of the nuclear membrane.
1994,
Pubmed
,
Xenbase
Li,
Isolation of ORC6, a component of the yeast origin recognition complex by a one-hybrid system.
1993,
Pubmed
Loo,
The origin recognition complex in silencing, cell cycle progression, and DNA replication.
1995,
Pubmed
Madine,
The nuclear envelope prevents reinitiation of replication by regulating the binding of MCM3 to chromatin in Xenopus egg extracts.
1995,
Pubmed
,
Xenbase
Madine,
MCM3 complex required for cell cycle regulation of DNA replication in vertebrate cells.
1995,
Pubmed
,
Xenbase
Maiorano,
Fission yeast cdc21, a member of the MCM protein family, is required for onset of S phase and is located in the nucleus throughout the cell cycle.
1996,
Pubmed
Miyake,
Identification of two Xenopus laevis genes, xMCM2 and xCDC46, with sequence homology to MCM genes involved in DNA replication.
1996,
Pubmed
,
Xenbase
Miyake,
Fission yeast genes nda1+ and nda4+, mutations of which lead to S-phase block, chromatin alteration and Ca2+ suppression, are members of the CDC46/MCM2 family.
1993,
Pubmed
Nishitani,
p65cdc18 plays a major role controlling the initiation of DNA replication in fission yeast.
1995,
Pubmed
Okishio,
Fission yeast Nda1 and Nda4, MCM homologs required for DNA replication, are constitutive nuclear proteins.
1996,
Pubmed
Rao,
Mammalian cell fusion: studies on the regulation of DNA synthesis and mitosis.
1970,
Pubmed
Someya,
The association and involvement of some members of the P1 protein family in a cell-free DNA replication of Xenopus eggs.
1995,
Pubmed
,
Xenbase
Thömmes,
Properties of the nuclear P1 protein, a mammalian homologue of the yeast Mcm3 replication protein.
1992,
Pubmed
Thömmes,
The RLF-M component of the replication licensing system forms complexes containing all six MCM/P1 polypeptides.
1997,
Pubmed
,
Xenbase
Todorov,
A human nuclear protein with sequence homology to a family of early S phase proteins is required for entry into S phase and for cell division.
1994,
Pubmed
Tye,
The MCM2-3-5 proteins: are they replication licensing factors?
1994,
Pubmed
Yan,
Cell cycle-regulated nuclear localization of MCM2 and MCM3, which are required for the initiation of DNA synthesis at chromosomal replication origins in yeast.
1993,
Pubmed
