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The assembly of photoreceptor outer segments into stacked discs is a complicated process, the precise regulation of which remains a mystery. It is known that the integrity of the outer segment is heavily dependent upon surrounding cell types including the retinal pigment epithelium and Müller cells; however the role played by Müller cells within this photoreceptor-specific process has not been fully explored. Using an RPE-deprived but otherwise intact Xenopus laevis eye rudiment preparation, we reveal that Müller cell involvement in outer segment assembly is dependent upon the stimulus provided to the retina. Pigment epithelium-derived factor is able to support proper membrane folding after inhibition of Müller cell metabolism by alpha-aminoadipic acid, while isopropyl beta-D-thiogalactoside, a permissive glycan, requires intact Müller cell function. These results demonstrate that both intrinsic and extrinsic redundant mechanisms exist to support the ability of photoreceptors to properly assemble their outer segments. Our study further suggests that the receptor for pigment epithelium-derived factor resides in photoreceptors themselves while that for permissive glycans is likely localized to Müller cells, which in turn communicate with photoreceptors to promote proper membrane assembly.
Anderson,
Morphological recovery in the reattached retina.
1986, Pubmed
Anderson,
Morphological recovery in the reattached retina.
1986,
Pubmed
Araque,
Glial modulation of synaptic transmission in culture.
2004,
Pubmed
Aymerich,
Evidence for pigment epithelium-derived factor receptors in the neural retina.
2001,
Pubmed
Cao,
Induction of c-fos and c-jun mRNA expression by basic fibroblast growth factor in cultured rat Müller cells.
1998,
Pubmed
Cao,
Pigment epithelium-derived factor protects cultured retinal neurons against hydrogen peroxide-induced cell death.
1999,
Pubmed
Cao,
Induction of basic fibroblast growth factor mRNA by basic fibroblast growth factor in Müller cells.
1997,
Pubmed
Cayouette,
Pigment epithelium-derived factor delays the death of photoreceptors in mouse models of inherited retinal degenerations.
1999,
Pubmed
Erickson,
Retinal detachment in the cat: the outer nuclear and outer plexiform layers.
1983,
Pubmed
Guérin,
Retinal reattachment of the primate macula. Photoreceptor recovery after short-term detachment.
1989,
Pubmed
Guérin,
Recovery of photoreceptor outer segment length and analysis of membrane assembly rates in regenerating primate photoreceptor outer segments.
1993,
Pubmed
Hollyfield,
Pigmented retinal epithelium involvement in photoreceptor development and function.
1974,
Pubmed
Imai,
Intraocular gene transfer of pigment epithelium-derived factor rescues photoreceptors from light-induced cell death.
2005,
Pubmed
Jablonski,
Closer look at lactose-mediated support of retinal morphogenesis.
2000,
Pubmed
,
Xenbase
Jablonski,
Targeted disruption of Müller cell metabolism induces photoreceptor dysmorphogenesis.
2000,
Pubmed
,
Xenbase
Jablonski,
Pigment epithelium-derived factor supports normal development of photoreceptor neurons and opsin expression after retinal pigment epithelium removal.
2000,
Pubmed
,
Xenbase
Jablonski,
Reliability assessment of a rod photoreceptor outer segment grading system.
2001,
Pubmed
,
Xenbase
Jablonski,
Pigment epithelium-derived factor supports normal Müller cell development and glutamine synthetase expression after removal of the retinal pigment epithelium.
2001,
Pubmed
,
Xenbase
Jablonski,
Lactose promotes organized photoreceptor outer segment assembly and preserves expression of photoreceptor proteins in retinal degeneration.
1999,
Pubmed
,
Xenbase
Lewis,
Opsin distribution and protein incorporation in photoreceptors after experimental retinal detachment.
1991,
Pubmed
Lin,
Synaptic signaling between neurons and glia.
2004,
Pubmed
Mori,
AAV-mediated gene transfer of pigment epithelium-derived factor inhibits choroidal neovascularization.
2002,
Pubmed
Newman,
Glial modulation of synaptic transmission in the retina.
2004,
Pubmed
Newman,
The Müller cell: a functional element of the retina.
1996,
Pubmed
Peng,
Alpha2-adrenergic agonists selectively activate extracellular signal-regulated kinases in Müller cells in vivo.
1998,
Pubmed
Reichenbach,
The Müller (glial) cell in normal and diseased retina: a case for single-cell electrophysiology.
1997,
Pubmed
Reichenbach,
What do retinal müller (glial) cells do for their neuronal 'small siblings'?
1993,
Pubmed
Robinson,
Müller cells in adult rabbit retinae: morphology, distribution and implications for function and development.
1990,
Pubmed
Stiemke,
Outer segment disc membrane assembly in the absence of the pigment epithelium: the effect of exogenous sugars.
1994,
Pubmed
,
Xenbase
Stiemke,
Photoreceptor outer segment development in Xenopus laevis: influence of the pigment epithelium.
1994,
Pubmed
,
Xenbase
Tepass,
Adherens junctions: new insight into assembly, modulation and function.
2002,
Pubmed
Turner,
A common progenitor for neurons and glia persists in rat retina late in development.
,
Pubmed
Ullian,
Role for glia in synaptogenesis.
2004,
Pubmed
Ullian,
Control of synapse number by glia.
2001,
Pubmed
Wahlin,
Neurotrophic factors cause activation of intracellular signaling pathways in Müller cells and other cells of the inner retina, but not photoreceptors.
2000,
Pubmed
Wang,
Permissive glycan support of photoreceptor outer segment assembly occurs via a non-metabolic mechanism.
2003,
Pubmed
,
Xenbase
Young,
The renewal of photoreceptor cell outer segments.
1967,
Pubmed
