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Mol Cell Biol
2005 Jan 01;251:241-9. doi: 10.1128/MCB.25.1.241-249.2005.
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The core histone N-terminal tail domains negatively regulate binding of transcription factor IIIA to a nucleosome containing a 5S RNA gene via a novel mechanism.
Yang Z
,
Zheng C
,
Thiriet C
,
Hayes JJ
.
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Reconstitution of a DNA fragment containing a 5S RNA gene from Xenopus borealis into a nucleosome greatly restricts binding of the primary 5S transcription factor, TFIIIA. Consistent with transcription experiments using reconstituted templates, removal of the histone tail domains stimulates TFIIIA binding to the 5S nucleosome greater than 100-fold. However, we show that tail removal increases the probability of 5S DNA unwrapping from the core histone surface by only approximately fivefold. Moreover, using site-specific histone-to-DNA cross-linking, we show that TFIIIA binding neither induces nor requires nucleosome movement. Binding studies with COOH-terminal deletion mutants of TFIIIA and 5S nucleosomes reconstituted with native and tailless core histones indicate that the core histone tail domains play a direct role in restricting the binding of TFIIIA. Deletion of only the COOH-terminal transcription activation domain dramatically stimulates TFIIIA binding to the native nucleosome, while further C-terminal deletions or removal of the tail domains does not lead to further increases in TFIIIA binding. We conclude that the unmodified core histone tail domains directly negatively influence TFIIIA binding to the nucleosome in a manner that requires the C-terminal transcription activation domain of TFIIIA. Our data suggest an additional mechanism by which the core histone tail domains regulate the binding of trans-acting factors in chromatin.
Allan,
Participation of core histone "tails" in the stabilization of the chromatin solenoid.
1982, Pubmed
Allan,
Participation of core histone "tails" in the stabilization of the chromatin solenoid.
1982,
Pubmed
Anderson,
Spontaneous access of proteins to buried nucleosomal DNA target sites occurs via a mechanism that is distinct from nucleosome translocation.
2002,
Pubmed
Aoyagi,
hSWI/SNF-catalyzed nucleosome sliding does not occur solely via a twist-diffusion mechanism.
2002,
Pubmed
,
Xenbase
Aoyagi,
Nucleosome sliding induced by the xMi-2 complex does not occur exclusively via a simple twist-diffusion mechanism.
2003,
Pubmed
,
Xenbase
Ausio,
Use of selectively trypsinized nucleosome core particles to analyze the role of the histone "tails" in the stabilization of the nucleosome.
1989,
Pubmed
Bauer,
Nucleosome structural changes due to acetylation.
1994,
Pubmed
,
Xenbase
Chafin,
Human DNA ligase I efficiently seals nicks in nucleosomes.
2000,
Pubmed
,
Xenbase
Clark,
Electrostatic mechanism of chromatin folding.
1990,
Pubmed
Clemens,
Definition of the binding sites of individual zinc fingers in the transcription factor IIIA-5S RNA gene complex.
1992,
Pubmed
,
Xenbase
Del Río,
High yield purification of active transcription factor IIIA expressed in E. coli.
1991,
Pubmed
,
Xenbase
Dong,
DNA and protein determinants of nucleosome positioning on sea urchin 5S rRNA gene sequences in vitro.
1990,
Pubmed
Dorigo,
Chromatin fiber folding: requirement for the histone H4 N-terminal tail.
2003,
Pubmed
,
Xenbase
Engelke,
Specific interaction of a purified transcription factor with an internal control region of 5S RNA genes.
1980,
Pubmed
,
Xenbase
Garcia-Ramirez,
Role of the histone "tails" in the folding of oligonucleosomes depleted of histone H1.
1992,
Pubmed
Hamiche,
Histone tails modulate nucleosome mobility and regulate ATP-dependent nucleosome sliding by NURF.
2001,
Pubmed
Hansen,
Influence of chromatin folding on transcription initiation and elongation by RNA polymerase III.
1992,
Pubmed
,
Xenbase
Hansen,
Conformational dynamics of the chromatin fiber in solution: determinants, mechanisms, and functions.
2002,
Pubmed
Hayes,
Structure of the TFIIIA-5 S DNA complex.
1992,
Pubmed
,
Xenbase
Hayes,
The structure of DNA in a nucleosome.
1990,
Pubmed
,
Xenbase
Hayes,
In vitro reconstitution and analysis of mononucleosomes containing defined DNAs and proteins.
1997,
Pubmed
,
Xenbase
Hayes,
Histones H2A/H2B inhibit the interaction of transcription factor IIIA with the Xenopus borealis somatic 5S RNA gene in a nucleosome.
1992,
Pubmed
,
Xenbase
Hayes,
A protein-protein interaction is essential for stable complex formation on a 5 S RNA gene.
1989,
Pubmed
,
Xenbase
Hayes,
Histone contributions to the structure of DNA in the nucleosome.
1991,
Pubmed
,
Xenbase
Howe,
Transcriptionally active Xenopus laevis somatic 5 S ribosomal RNA genes are packaged with hyperacetylated histone H4, whereas transcriptionally silent oocyte genes are not.
1998,
Pubmed
,
Xenbase
Jenuwein,
Translating the histone code.
2001,
Pubmed
Kassabov,
High-resolution mapping of changes in histone-DNA contacts of nucleosomes remodeled by ISW2.
2002,
Pubmed
Lassar,
Transcription of class III genes: formation of preinitiation complexes.
1983,
Pubmed
,
Xenbase
Lee,
Targeted cross-linking and DNA cleavage within model chromatin complexes.
1999,
Pubmed
,
Xenbase
Lee,
A positive role for histone acetylation in transcription factor access to nucleosomal DNA.
1993,
Pubmed
,
Xenbase
Lefebvre,
Binding of retinoic acid receptor heterodimers to DNA. A role for histones NH2 termini.
1998,
Pubmed
Lomvardas,
Nucleosome sliding via TBP DNA binding in vivo.
2001,
Pubmed
Nolte,
Differing roles for zinc fingers in DNA recognition: structure of a six-finger transcription factor IIIA complex.
1998,
Pubmed
,
Xenbase
Panetta,
Differential nucleosome positioning on Xenopus oocyte and somatic 5 S RNA genes determines both TFIIIA and H1 binding: a mechanism for selective H1 repression.
1998,
Pubmed
,
Xenbase
Polach,
Effects of core histone tail domains on the equilibrium constants for dynamic DNA site accessibility in nucleosomes.
2000,
Pubmed
Polach,
Mechanism of protein access to specific DNA sequences in chromatin: a dynamic equilibrium model for gene regulation.
1995,
Pubmed
Rhodes,
Structural analysis of a triple complex between the histone octamer, a Xenopus gene for 5S RNA and transcription factor IIIA.
1985,
Pubmed
,
Xenbase
Roth,
Histone acetyltransferases.
2001,
Pubmed
Schwarz,
Reversible oligonucleosome self-association: dependence on divalent cations and core histone tail domains.
1996,
Pubmed
Simon,
A new procedure for purifying histone pairs H2A + H2B and H3 + H4 from chromatin using hydroxylapatite.
1979,
Pubmed
Strahl,
The language of covalent histone modifications.
2000,
Pubmed
Thiriet,
Functionally relevant histone-DNA interactions extend beyond the classically defined nucleosome core region.
1998,
Pubmed
,
Xenbase
Tse,
Disruption of higher-order folding by core histone acetylation dramatically enhances transcription of nucleosomal arrays by RNA polymerase III.
1998,
Pubmed
,
Xenbase
Vettese-Dadey,
Acetylation of histone H4 plays a primary role in enhancing transcription factor binding to nucleosomal DNA in vitro.
1996,
Pubmed
Vettese-Dadey,
Role of the histone amino termini in facilitated binding of a transcription factor, GAL4-AH, to nucleosome cores.
1994,
Pubmed
Vitolo,
The H3-H4 N-terminal tail domains are the primary mediators of transcription factor IIIA access to 5S DNA within a nucleosome.
2000,
Pubmed
,
Xenbase
Vitolo,
Structural features of transcription factor IIIA bound to a nucleosome in solution.
2004,
Pubmed
,
Xenbase
Vrana,
Mapping functional regions of transcription factor TFIIIA.
1988,
Pubmed
,
Xenbase
Wolffe,
Transcription factor access to DNA in the nucleosome.
1993,
Pubmed
,
Xenbase
Wolffe,
Developmental regulation of two 5S ribosomal RNA genes.
1988,
Pubmed
,
Xenbase
Yang,
Large scale preparation of nucleosomes containing site-specifically chemically modified histones lacking the core histone tail domains.
2004,
Pubmed
