Jindrichova M , Lansdell SJ , Millar NS .

WT085141 Wellcome Trust , Wellcome Trust

	Figure 1. Electrophysiological characterization of α7 nAChRs expressed in Xenopus oocytes in response to acetylcholine.Bar charts illustrate responses (mean ± SEM) from α7 nAChRs expressed in Xenopus oocytes in response to a maximal (3 mM) and EC50 (100 µM) concentration of acetylcholine (A and B, respectively) at room temperature (RT; 21°C), higher temperature (37°C) and lower temperature (4°C). Data are means of 7–11 responses, each from a different oocyte, in which responses obtained at either 4°C or 37°C are normalized to responses obtained from the same oocyte at RT. C) Representative traces illustrating responses obtained at RT (upper trace) and 4°C (lower trace) from a single oocyte. D) Representative traces illustrating responses obtained at RT (upper trace) and 37°C (lower trace) from a single oocyte.
	Figure 2. Electrophysiological characterization of α4β2 nAChRs expressed in Xenopus oocytes in response to acetylcholine.Bar charts illustrate responses (mean ± SEM) from α4β2 nAChRs expressed in Xenopus oocytes in response to a maximal (1 mM) concentration of acetylcholine in either calcium-contanining (A) or barium-containing Ringer solution (B) at room temperature (RT; 21°C), higher temperature (37°C) and lower temperature (4°C). Data are means of 6–9 responses, each from a different oocyte, in which responses obtained at either 4°C or 37°C are normalized to responses obtained from the same oocyte at RT. C) Representative traces illustrating responses obtained at RT (upper trace) and 4°C (lower trace) from a single oocyte. D) Representative traces illustrating responses obtained at RT (upper trace) and 37°C (lower trace) from a single oocyte. Representative traces are from calcium-containing saline but similar responses were obtained with barium-containing saline.
	Figure 3. Electrophysiological characterization of α7L247T nAChRs expressed in Xenopus oocytes in response to acetylcholine.Bar charts illustrate responses (mean ± SEM) from α7L247T nAChRs expressed in Xenopus oocytes in response to a maximal (30 µM) and EC50 (0.4 µM) concentration of acetylcholine (A and B, respectively) at room temperature (RT; 21°C), higher temperature (37°C) and lower temperature (4°C). Data are means of 5–9 responses, each from a different oocyte, in which responses obtained at either 4°C or 37°C are normalized to responses obtained from the same oocyte at RT. C) Representative traces illustrating responses obtained at RT (upper trace) and 4°C (lower trace) from a single oocyte. D) Representative traces illustrating responses obtained at RT (upper trace) and 37°C (lower trace) from a single oocyte.
	Figure 4. Electrophysiological characterization of α7 nAChRs expressed in Xenopus oocytes in response to 4BP-TQS.A) A bar chart illustrates responses (mean ± SEM) from α7 nAChRs expressed in Xenopus oocytes in response to a maximal (10 µM) concentration of the allosteric agonist 4BP-TQS at room temperature (RT; 21°C), higher temperature (37°C) and lower temperature (4°C). Data are means of 5–22 responses, each from a different oocyte, in which responses obtained at either 4°C or 37°C are normalized to responses obtained from the same oocyte at RT. B) Representative traces illustrating responses obtained at RT (upper trace) and 4°C (lower trace) from a single oocyte. C) Representative traces illustrating responses obtained at RT (upper trace) and 37°C (lower trace) from a single oocyte.
	Figure 5. Electrophysiological characterization of nAChRs expressed in Xenopus oocytes examined at different temperatures.Representative traces are shown illustrating responses obtained at RT (black), 4°C (blue) and 37°C (red). Current traces obtained at each temperature have been normalized to the same peak response. In each case, the response showing the fastest rate of desensitization was observed at 37°C and the slowest rate of desensitization was observed at 4°C. Responses are from α7 nAChRs with 3 mM acetylcholine (A), α4β2 nAChRs with 1 mM acetylcholine in calcium-containing saline (B), α7L247T nAChRs with 30 µM acetylcholine (C) and α7 nAChRs with 10 µM 4BP-TQS (D). Rates of receptor deactivation after removal of agonist were also influenced in a consistent manner by changes in temperature (faster at 37°C and slower at 4°C). Representative traces from α7L247T nAChRs with 30 µM acetylcholine are illustrated (E) and are typical of results from all receptor/agonist combinations studied (see Tables 1 and 2 for details).

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