Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Proc Natl Acad Sci U S A
1987 Nov 01;8422:7896-900.
Show Gene links
Show Anatomy links
Selective elimination of mRNAs in vivo: complementary oligodeoxynucleotides promote RNA degradation by an RNase H-like activity.
Dash P
,
Lotan I
,
Knapp M
,
Kandel ER
,
Goelet P
.
???displayArticle.abstract???
Oligodeoxynucleotides lead to translation arrest of complementary mRNAs in the wheat germ translation system by a degradation of the mRNA. In an attempt to develop an effective reverse genetic approach in vivo, we demonstrate that injection of short (15- to 30-nucleotide) oligonucleotides into Xenopus oocytes leads to complete degradation of both injected and endogenous mRNAs by means of an RNase H-like activity.
Bass,
A developmentally regulated activity that unwinds RNA duplexes.
1987, Pubmed,
Xenbase
Bass,
A developmentally regulated activity that unwinds RNA duplexes.
1987,
Pubmed
,
Xenbase
Berk,
Sizing and mapping of early adenovirus mRNAs by gel electrophoresis of S1 endonuclease-digested hybrids.
1977,
Pubmed
Cazenave,
Enzymatic amplification of translation inhibition of rabbit beta-globin mRNA mediated by anti-messenger oligodeoxynucleotides covalently linked to intercalating agents.
1987,
Pubmed
,
Xenbase
Cheng,
Isolation and nucleotide sequence of the rabbit globin gene cluster psi zeta-alpha 1-psi alpha. Absence of a pair of alpha-globin genes evolving in concert.
1986,
Pubmed
Chien,
Isolation and characterization of calmodulin genes from Xenopus laevis.
1984,
Pubmed
,
Xenbase
Dascal,
Expression and modulation of voltage-gated calcium channels after RNA injection in Xenopus oocytes.
1986,
Pubmed
,
Xenbase
Ecker,
Inhibition of gene expression in plant cells by expression of antisense RNA.
1986,
Pubmed
Gubler,
A simple and very efficient method for generating cDNA libraries.
1983,
Pubmed
Gurdon,
Use of frog eggs and oocytes for the study of messenger RNA and its translation in living cells.
1971,
Pubmed
,
Xenbase
Harland,
Translation of mRNA injected into Xenopus oocytes is specifically inhibited by antisense RNA.
1985,
Pubmed
,
Xenbase
Izant,
Constitutive and conditional suppression of exogenous and endogenous genes by anti-sense RNA.
1985,
Pubmed
,
Xenbase
Izant,
Inhibition of thymidine kinase gene expression by anti-sense RNA: a molecular approach to genetic analysis.
1984,
Pubmed
Kawasaki,
Quantitative hybridization-arrest of mRNA in Xenopus oocytes using single-stranded complementary DNA or oligonucleotide probes.
1985,
Pubmed
,
Xenbase
Kim,
Stable reduction of thymidine kinase activity in cells expressing high levels of anti-sense RNA.
1985,
Pubmed
Melton,
Injected anti-sense RNAs specifically block messenger RNA translation in vivo.
1985,
Pubmed
,
Xenbase
Minshull,
The use of single-stranded DNA and RNase H to promote quantitative 'hybrid arrest of translation' of mRNA/DNA hybrids in reticulocyte lysate cell-free translations.
1986,
Pubmed
Palmiter,
Magnesium precipitation of ribonucleoprotein complexes. Expedient techniques for the isolation of undergraded polysomes and messenger ribonucleic acid.
1974,
Pubmed
Paterson,
Structural gene identification and mapping by DNA-mRNA hybrid-arrested cell-free translation.
1977,
Pubmed
Rebagliati,
Antisense RNA injections in fertilized frog eggs reveal an RNA duplex unwinding activity.
1987,
Pubmed
,
Xenbase
Rohrbaugh,
Transcription unit of the rabbit beta 1 globin gene.
1985,
Pubmed
Rosenberg,
Production of phenocopies by Krüppel antisense RNA injection into Drosophila embryos.
,
Pubmed
Sanger,
Cloning in single-stranded bacteriophage as an aid to rapid DNA sequencing.
1980,
Pubmed
Smith,
Antiviral effect of an oligo(nucleoside methylphosphonate) complementary to the splice junction of herpes simplex virus type 1 immediate early pre-mRNAs 4 and 5.
1986,
Pubmed
Stein,
Enzyme from calf thymus degrading the RNA moiety of DNA-RNA Hybrids: effect on DNA-dependent RNA polymerase.
1969,
Pubmed
Thomas,
Hybridization of denatured RNA and small DNA fragments transferred to nitrocellulose.
1980,
Pubmed
Zamecnik,
Inhibition of replication and expression of human T-cell lymphotropic virus type III in cultured cells by exogenous synthetic oligonucleotides complementary to viral RNA.
1986,
Pubmed
