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Expression of the rabbit intestinal N2 Na+/nucleoside transporter in Xenopus laevis oocytes.
Jarvis SM
,
Griffith DA
.
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Polyadenylated [poly(A)+] mRNA isolated from rabbit small-intestinal mucosa was injected into Xenopus laevis oocytes, and expression of the N2 Na+/nucleoside co-transporter was assayed by measuring Na(+)-dependent thymidine uptake. Expression of Na(+)-dependent thymidine uptake steadily increased after mRNA injection and was on average increased 11-fold by day 6 over background. Na(+)-dependent thymidine uptake was saturable (apparent Km approximately 30 microM at 22 degrees C) and inhibited by uridine and cytidine, but not by guanosine and inosine. These properties of the expressed thymidine transport strongly suggest that the epithelial N2 Na+/nucleoside co-transporter can be expressed in X. laevis oocytes.
Baer,
Sodium-dependent and inhibitor-insensitive uptake of adenosine by mouse peritoneal exudate cells.
1990, Pubmed
Baer,
Sodium-dependent and inhibitor-insensitive uptake of adenosine by mouse peritoneal exudate cells.
1990,
Pubmed
Betcher,
Sodium-adenosine cotransport in brush-border membranes from rabbit ileum.
1990,
Pubmed
Coady,
Expression of mammalian renal transporters in Xenopus laevis oocytes.
1990,
Pubmed
,
Xenbase
Darnowski,
Concentrative uridine transport by murine splenocytes: kinetics, substrate specificity, and sodium dependency.
1987,
Pubmed
Jakobs,
Expression of sodium-linked nucleoside transport activity in monolayer cultures of IEC-6 intestinal epithelial cells.
1990,
Pubmed
Jarvis,
Characterization of sodium-dependent nucleoside transport in rabbit intestinal brush-border membrane vesicles.
1989,
Pubmed
Lee,
Induction of the differentiation of HL-60 cells by phorbol 12-myristate 13-acetate activates a Na(+)-dependent uridine-transport system. Involvement of protein kinase C.
1991,
Pubmed
Lee,
Transport characteristics of renal brush border Na(+)- and K(+)-dependent uridine carriers.
1990,
Pubmed
Le Hir,
Uphill transport of pyrimidine nucleosides in renal brush border vesicles.
1985,
Pubmed
Le Hir,
Evidence for separate carriers for purine nucleosides and for pyrimidine nucleosides in the renal brush border membrane.
1990,
Pubmed
Plagemann,
Nucleoside and nucleobase transport in animal cells.
1988,
Pubmed
Plagemann,
Na(+)-dependent, active nucleoside transport in mouse spleen lymphocytes, leukemia cells, fibroblasts and macrophages, but not in equivalent human or pig cells; dipyridamole enhances nucleoside salvage by cells with both active and facilitated transport.
1990,
Pubmed
Schwenk,
Uridine uptake by isolated intestinal epithelial cells of guinea pig.
1984,
Pubmed
Sigel,
Use of Xenopus oocytes for the functional expression of plasma membrane proteins.
1990,
Pubmed
,
Xenbase
Tate,
Secreted alkaline phosphatase: an internal standard for expression of injected mRNAs in the Xenopus oocyte.
1990,
Pubmed
,
Xenbase
Vijayalakshmi,
Sodium-dependent nucleoside transport in mouse intestinal epithelial cells. Two transport systems with differing substrate specificities.
1988,
Pubmed
Williams,
Multiple sodium-dependent nucleoside transport systems in bovine renal brush-border membrane vesicles.
1991,
Pubmed
