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Planta
2012 Feb 01;2352:311-23. doi: 10.1007/s00425-011-1508-7.
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AtPTR4 and AtPTR6 are differentially expressed, tonoplast-localized members of the peptide transporter/nitrate transporter 1 (PTR/NRT1) family.
Weichert A
,
Brinkmann C
,
Komarova NY
,
Dietrich D
,
Thor K
,
Meier S
,
Suter Grotemeyer M
,
Rentsch D
.
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Members of the peptide transporter/nitrate transporter 1 (PTR/NRT1) family in plants transport a variety of substrates like nitrate, di- and tripepetides, auxin and carboxylates. We isolated two members of this family from Arabidopsis, AtPTR4 and AtPTR6, which are highly homologous to the characterized di- and tripeptide transporters AtPTR1, AtPTR2 and AtPTR5. All known substrates of members of the PTR/NRT1 family were tested using heterologous expression in Saccharomyces cerevisiae mutants and oocytes of Xenopus laevis, but none could be identified as substrate of AtPTR4 or AtPTR6. AtPTR4 and AtPTR6 show distinct expression patterns, while AtPTR4 is expressed in the vasculature of the plants, AtPTR6 is highly expressed in pollen and during senescence. Phylogenetic analyses revealed that AtPTR2, 4 and 6 belong to one clade of subgoup II, whereas AtPTR1 and 5 are found in a second clade. Like AtPTR2, AtPTR4-GFP and AtPTR6-GFP fusion proteins are localized at the tonoplast. Vacuolar localization was corroborated by co-localization of AtPTR2-YFP with the tonoplast marker protein GFP-AtTIP2;1 and AtTIP1;1-GFP. This indicates that the two clades reflect different intracellular localization at the tonoplast (AtPTR2, 4, 6) and plasma membrane (AtPTR1, 5), respectively.
Abel,
Transient transformation of Arabidopsis leaf protoplasts: a versatile experimental system to study gene expression.
1994, Pubmed
Abel,
Transient transformation of Arabidopsis leaf protoplasts: a versatile experimental system to study gene expression.
1994,
Pubmed
Alexandrov,
Features of Arabidopsis genes and genome discovered using full-length cDNAs.
2006,
Pubmed
Baukrowitz,
Inward rectification in KATP channels: a pH switch in the pore.
1999,
Pubmed
Bock,
Integrating membrane transport with male gametophyte development and function through transcriptomics.
2006,
Pubmed
Boursiac,
Early effects of salinity on water transport in Arabidopsis roots. Molecular and cellular features of aquaporin expression.
2005,
Pubmed
Campalans,
Identification of differentially expressed genes by the cDNA-AFLP technique during dehydration of almond (Prunus amygdalus).
2001,
Pubmed
Carter,
The vegetative vacuole proteome of Arabidopsis thaliana reveals predicted and unexpected proteins.
2004,
Pubmed
Chiang,
Mechanisms and functional properties of two peptide transporters, AtPTR2 and fPTR2.
2004,
Pubmed
,
Xenbase
Clough,
Floral dip: a simplified method for Agrobacterium-mediated transformation of Arabidopsis thaliana.
1998,
Pubmed
Dietrich,
AtPTR1, a plasma membrane peptide transporter expressed during seed germination and in vascular tissue of Arabidopsis.
2004,
Pubmed
,
Xenbase
Dohmen,
An efficient transformation procedure enabling long-term storage of competent cells of various yeast genera.
1991,
Pubmed
Dunkley,
Mapping the Arabidopsis organelle proteome.
2006,
Pubmed
Endler,
Identification of a vacuolar sucrose transporter in barley and Arabidopsis mesophyll cells by a tonoplast proteomic approach.
2006,
Pubmed
Frommer,
Cloning of an Arabidopsis histidine transporting protein related to nitrate and peptide transporters.
1994,
Pubmed
Grudkowska,
Multifunctional role of plant cysteine proteinases.
2004,
Pubmed
Hammes,
Functional properties of the Arabidopsis peptide transporters AtPTR1 and AtPTR5.
2010,
Pubmed
,
Xenbase
Herman,
Protein storage bodies and vacuoles.
1999,
Pubmed
Higgins,
Peptide transport by germinating barley embryos: Uptake of physiological di- and oligopeptides.
1978,
Pubmed
Hörtensteiner,
Nitrogen metabolism and remobilization during senescence.
2002,
Pubmed
Hruz,
Genevestigator v3: a reference expression database for the meta-analysis of transcriptomes.
2008,
Pubmed
Jamai,
Proton-Peptide Co-Transport in Broad Bean Leaf Tissues.
1994,
Pubmed
Jeong,
A nodule-specific dicarboxylate transporter from alder is a member of the peptide transporter family.
2004,
Pubmed
,
Xenbase
Karim,
AtPTR3, a wound-induced peptide transporter needed for defence against virulent bacterial pathogens in Arabidopsis.
2007,
Pubmed
Komarova,
AtPTR1 and AtPTR5 transport dipeptides in planta.
2008,
Pubmed
,
Xenbase
Krouk,
Nitrate-regulated auxin transport by NRT1.1 defines a mechanism for nutrient sensing in plants.
2010,
Pubmed
,
Xenbase
Li,
The Arabidopsis nitrate transporter NRT1.8 functions in nitrate removal from the xylem sap and mediates cadmium tolerance.
2010,
Pubmed
,
Xenbase
Lin,
Cloning and functional characterization of a constitutively expressed nitrate transporter gene, OsNRT1, from rice.
2000,
Pubmed
,
Xenbase
Lin,
Mutation of the Arabidopsis NRT1.5 nitrate transporter causes defective root-to-shoot nitrate transport.
2008,
Pubmed
,
Xenbase
Martinoia,
Vacuolar transporters and their essential role in plant metabolism.
2007,
Pubmed
Marty,
Plant vacuoles.
1999,
Pubmed
Mignone,
Untranslated regions of mRNAs.
2002,
Pubmed
Miranda,
Peptide and amino acid transporters are differentially regulated during seed development and germination in faba bean.
2003,
Pubmed
Müntz,
Protein dynamics and proteolysis in plant vacuoles.
2007,
Pubmed
Näsholm,
Uptake of organic nitrogen by plants.
2009,
Pubmed
Parrott,
Steam-girdling of barley (Hordeum vulgare) leaves leads to carbohydrate accumulation and accelerated leaf senescence, facilitating transcriptomic analysis of senescence-associated genes.
2007,
Pubmed
Paungfoo-Lonhienne,
Nitrogen affects cluster root formation and expression of putative peptide transporters.
2009,
Pubmed
Paungfoo-Lonhienne,
Plants can use protein as a nitrogen source without assistance from other organisms.
2008,
Pubmed
Ramos,
Characterization of a transport activity for long-chain peptides in barley mesophyll vacuoles.
2011,
Pubmed
Rentsch,
NTR1 encodes a high affinity oligopeptide transporter in Arabidopsis.
1995,
Pubmed
Rentsch,
Transporters for uptake and allocation of organic nitrogen compounds in plants.
2007,
Pubmed
Schwacke,
ARAMEMNON, a novel database for Arabidopsis integral membrane proteins.
2003,
Pubmed
Shimaoka,
Isolation of intact vacuoles and proteomic analysis of tonoplast from suspension-cultured cells of Arabidopsis thaliana.
2004,
Pubmed
Song,
Cloning of a second Arabidopsis peptide transport gene.
1996,
Pubmed
Song,
Engineering tolerance and accumulation of lead and cadmium in transgenic plants.
2003,
Pubmed
Swarbreck,
The Arabidopsis Information Resource (TAIR): gene structure and function annotation.
2008,
Pubmed
Tegeder,
Uptake and partitioning of amino acids and peptides.
2010,
Pubmed
Tsay,
Nitrate transporters and peptide transporters.
2007,
Pubmed
Wang,
Transcriptome analyses show changes in gene expression to accompany pollen germination and tube growth in Arabidopsis.
2008,
Pubmed
Waterworth,
The barley scutellar peptide transporter: biochemical characterization and localization to the plasma membrane.
2000,
Pubmed
,
Xenbase
West,
Cloning and functional characterisation of a peptide transporter expressed in the scutellum of barley grain during the early stages of germination.
1998,
Pubmed
,
Xenbase
Winter,
An "Electronic Fluorescent Pictograph" browser for exploring and analyzing large-scale biological data sets.
2007,
Pubmed
WRIGHT,
Amino acid uptake by plant roots.
1962,
Pubmed
Xiang,
A mini binary vector series for plant transformation.
1999,
Pubmed
Yamashita,
Cloning and functional expression of a brain peptide/histidine transporter.
1997,
Pubmed
,
Xenbase
