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Fodrin (nonerythroid spectrin) and its associated proteins have been previously implicated in the establishment of specialized membrane-cytoskeletal domains in differentiating cells. Using antiserum which is monospecific for the alpha-subunit of fodrin, we demonstrate that alpha-fodrin is present in oocytes and adult tissues of Xenopus laevis. Analyses of the de novo synthesis of alpha-fodrin during embryonic development reveal that alpha-fodrin is synthesized in oocytes, but not during early development. To investigate the level of control of alpha-fodrin expression, we isolated two cDNA clones for oocyte alpha-fodrin. The oocyte cDNA clones were identified as encoding portions of alpha-fodrin based on DNA sequence analysis and on the comparison of the predicted amino acid sequence of the cDNAs with the known sequence of human erythrocyte alpha-spectrin. The Xenopus alpha-fodrin cDNAs hybridize to a transcript of approximately 9 kb on RNA blots, and probably to a single gene type on genomic DNA blots. Both RNA blot analyses and S1 nuclease protection assays with the Xenopus alpha-fodrin cDNAs demonstrate that the observed decline in the de novo synthesis of alpha-fodrin polypeptides is controlled by a dramatic decrease in the abundance of alpha-fodrin transcripts after fertilization. In contrast, levels of actin transcripts do not decrease during this period. Inasmuch as steady-state levels of alpha-fodrin transcripts rise by the neurula stage of development, these results suggest that the synthesis of alpha-fodrin polypeptides during embryonic development of Xenopus is regulated, rather than constitutive, and that the primary level of control is the steady-state abundance of mRNA.
Aviv,
Purification of biologically active globin messenger RNA by chromatography on oligothymidylic acid-cellulose.
1972, Pubmed
Aviv,
Purification of biologically active globin messenger RNA by chromatography on oligothymidylic acid-cellulose.
1972,
Pubmed
Bluemink,
The plasma-membrance IMP pattern as related to animal/vegetal polarity in the amphibian egg.
1978,
Pubmed
,
Xenbase
Chou,
Prediction of the secondary structure of proteins from their amino acid sequence.
1978,
Pubmed
Chou,
Empirical predictions of protein conformation.
1978,
Pubmed
Colot,
Behavior of individual maternal pA+ RNAs during embryogenesis of Xenopus laevis.
1982,
Pubmed
,
Xenbase
Dworkin,
Use of a cloned library for the study of abundant poly(A)+RNA during Xenopus laevis development.
1980,
Pubmed
,
Xenbase
Dworkin,
Mitochondrial RNAs are abundant in the poly(A)+RNA population of early frog embryos.
1981,
Pubmed
,
Xenbase
Feinberg,
A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity.
1983,
Pubmed
Franke,
Distribution and mode of arrangement of microfilamentous structures and actin in the cortex of the amphibian oocyte.
1976,
Pubmed
Franz,
Intermediate-size filaments in a germ cell: Expression of cytokeratins in oocytes and eggs of the frog Xenopus.
1983,
Pubmed
,
Xenbase
Glenney,
Comparison of spectrin isolated from erythroid and non-erythroid sources.
1984,
Pubmed
Godsave,
Intermediate filaments in the Xenopus oocyte: the appearance and distribution of cytokeratin-containing filaments.
1984,
Pubmed
,
Xenbase
Godsave,
Oocytes and early embryos of Xenopus laevis contain intermediate filaments which react with anti-mammalian vimentin antibodies.
1984,
Pubmed
,
Xenbase
Gurdon,
The use of Xenopus oocytes for the expression of cloned genes.
1983,
Pubmed
,
Xenbase
Heikkila,
Acquisition of the heat-shock response and thermotolerance during early development of Xenopus laevis.
1985,
Pubmed
,
Xenbase
Koenig,
A regional analysis of alpha-spectrin in the isolated Mauthner neuron and in isolated axons of the goldfish and rabbit.
1985,
Pubmed
Krieg,
Developmental regulation of a gastrula-specific gene injected into fertilized Xenopus eggs.
1985,
Pubmed
,
Xenbase
Lazarides,
Segregation of two spectrin forms in the chicken optic system: a mechanism for establishing restricted membrane-cytoskeletal domains in neurons.
1984,
Pubmed
Levine,
Redistribution of fodrin (a component of the cortical cytoplasm) accompanying capping of cell surface molecules.
1983,
Pubmed
Levine,
Fodrin: axonally transported polypeptides associated with the internal periphery of many cells.
1981,
Pubmed
Minty,
Mouse actin messenger RNAs. Construction and characterization of a recombinant plasmid molecule containing a complementary DNA transcript of mouse alpha-actin mRNA.
1981,
Pubmed
Moon,
Tissue-specific expression of distinct spectrin and ankyrin transcripts in erythroid and nonerythroid cells.
1985,
Pubmed
Moon,
An assessment of the masked message hypothesis: sea urchin egg messenger ribonucleoprotein complexes are efficient templates for in vitro protein synthesis.
1982,
Pubmed
Müller,
A homeo-box-containing gene expressed during oogenesis in Xenopus.
1984,
Pubmed
,
Xenbase
Nelson,
Involvement of spectrin in cell-surface receptor capping in lymphocytes.
1983,
Pubmed
Newport,
A major developmental transition in early Xenopus embryos: I. characterization and timing of cellular changes at the midblastula stage.
1982,
Pubmed
,
Xenbase
Rebagliati,
Identification and cloning of localized maternal RNAs from Xenopus eggs.
1985,
Pubmed
,
Xenbase
Repasky,
Widespread occurrence of avian spectrin in nonerythroid cells.
1982,
Pubmed
Ruderman,
Modulations of histone messenger RNA during the early development of Xenopus laevis.
1979,
Pubmed
,
Xenbase
Sanger,
Cloning in single-stranded bacteriophage as an aid to rapid DNA sequencing.
1980,
Pubmed
Schatten,
Localization of fodrin during fertilization and early development of sea urchins and mice.
1986,
Pubmed
Sobel,
Changes in the distribution of a spectrin-like protein during development of the preimplantation mouse embryo.
1985,
Pubmed
Speicher,
Erythrocyte spectrin is comprised of many homologous triple helical segments.
,
Pubmed
Vacquier,
Dynamic changes of the egg cortex.
1981,
Pubmed
Wasserman,
Protein synthesis during maturation promoting factor- and progesterone-induced maturation in Xenopus oocytes.
1982,
Pubmed
,
Xenbase
